Pharmacokinetic study of an anti‐trypanosome agent with different formulations and administration routes in mice by HPLC–MS/MS

Previously compound 12 showed great anti‐trypanosome activity without toxicity in an in vivo study. In the current study, a sensitive and rapid high‐performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method was developed and validated to investigate its pharmacokinetics in mouse plasma. A protein precipitation method was applied to extract the compound, and it was then separated using a Kinetex C 18 column with mobile phase...