The relationship between crosslinking structure and silk fibroin scaffold performance for soft tissue engineering

Published on Jul 1, 2021in International Journal of Biological Macromolecules5.162
· DOI :10.1016/J.IJBIOMAC.2021.05.058
Zhinan Mao3
Estimated H-index: 3
(Beihang University),
Xuewei Bi3
Estimated H-index: 3
(Beihang University)
+ 6 AuthorsSujun Wu16
Estimated H-index: 16
(Beihang University)
Source
Abstract
Abstract Biologically active scaffolds with tunable mechano- and bio-performance remain desirable for soft tissue engineering. Previously, highly elastic and robust silk fibroin (SF) scaffolds were prepared via cryogelation. In order to get more insight into the role of ethylene glycol diglycidyl ether (EGDE) on the structure and properties of SF scaffolds, we investigated the fate of SF scaffolds with different usages of the crosslinking agent in vitro and in vivo. Although SF scaffolds with varied EGDE contents showed similar micro-morphology, increasing EGDE from 1 mmol/g to 5 mmol/g resulted in firstly increased and later decreased content of β-sheet conformation, and linearly increased tensile modulus and decreased elasticity. The dual-crosslinked SF scaffolds with EGDE up to 5 mmol/g did not show in vitro cytotoxicity for NIH3T3 fibroblasts. In vivo subcutaneous implantation of SF scaffolds with
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