The relationship between crosslinking structure and silk fibroin scaffold performance for soft tissue engineering

Published on Jul 1, 2021in International Journal of Biological Macromolecules5.162
· DOI :10.1016/J.IJBIOMAC.2021.05.058
Zhinan Mao3
Estimated H-index: 3
(Beihang University),
Xuewei Bi3
Estimated H-index: 3
(Beihang University)
+ 6 AuthorsSujun Wu16
Estimated H-index: 16
(Beihang University)
Abstract Biologically active scaffolds with tunable mechano- and bio-performance remain desirable for soft tissue engineering. Previously, highly elastic and robust silk fibroin (SF) scaffolds were prepared via cryogelation. In order to get more insight into the role of ethylene glycol diglycidyl ether (EGDE) on the structure and properties of SF scaffolds, we investigated the fate of SF scaffolds with different usages of the crosslinking agent in vitro and in vivo. Although SF scaffolds with varied EGDE contents showed similar micro-morphology, increasing EGDE from 1 mmol/g to 5 mmol/g resulted in firstly increased and later decreased content of β-sheet conformation, and linearly increased tensile modulus and decreased elasticity. The dual-crosslinked SF scaffolds with EGDE up to 5 mmol/g did not show in vitro cytotoxicity for NIH3T3 fibroblasts. In vivo subcutaneous implantation of SF scaffolds with
#1Donghui Zhang (ECUST: East China University of Science and Technology)H-Index: 4
#2Qi Chen (ECUST: East China University of Science and Technology)H-Index: 4
Last. Runhui Liu (ECUST: East China University of Science and Technology)H-Index: 20
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Last. Xiaoming Li (Beihang University)H-Index: 105
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Naturally derived extracellular matrix scaffolds can effectively promote tissue repair and regeneration due to its remarkable bioactivity. However, its rapid degradation leads to the decrease of mechanical retention and the failure of physical support in vivo which limits its applications. In this paper, we modified a classic extracellular matrix scaffold-small intestinal submucosa (SIS) by silk fibroin (SF) layer-by-layer (LbL) assembly to replace the existing chemical crosslinking methods for ...
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#1Zhinan Mao (Beihang University)H-Index: 1
#2Xuewei Bi (Beihang University)H-Index: 1
Last. Sujun Wu (Beihang University)H-Index: 16
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Silk biomaterials with tunable mechanical properties and biological properties are of special importance for tissue engineering. Here, we fabricated silk fibroin (SF, from Bombyx mori silk) scaffol...
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#2Yanbing Liang (Beihang University)H-Index: 1
Last. Yubo Fan (Beihang University)H-Index: 64
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Summary Graphene-based substrates are emerging as a promising functional platform for biomedical applications. Although dispersible graphene sheets have been demonstrated to be biodegradable, their assembled macroscopic architectures are biopersistent because of strong π-π interactions. In this study, we developed a nacre-inspired graphene-silk nanocomposite film by vacuum filtration with a subsequent green chemical reduction procedure. The “brick-and-mortar” architecture not only ensures the me...
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#2Biman B. Mandal (IITG: Indian Institute of Technology Guwahati)H-Index: 38
Abstract Silk biomaterials are known for biomedical and tissue engineering applications including drug delivery and implantable devices owing to their biocompatible and a wide range of ideal physico-chemical properties. Herein, we present a critical overview of the progress of silk-based matrices in skin regeneration therapeutics with an emphasis on recent innovations and scientific findings. Beginning with a brief description of numerous varieties of silks, the review summarizes our current und...
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Biodegradable materials, including natural and synthetic polymers and hydrolyzable metals, constitute the main components of temporary, implantable medical devices. Besides the intrinsic properties of the materials, the most critical factor determining the successful clinical outcome of implantable and degradable devices is the host response, particularly the immune response, which largely depends on the material features and degradation mechanisms. In this Review, we first survey the state of t...
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Abstract Glycosaminoglycan (GAG)-based, biohybrid hydrogels offering far-reaching control over their physical and biomolecular signaling properties have been successfully used in various cell and tissue culture applications. To explore the suitability of the materials for in vivo use, we herein studied the host reaction to in situ-assembling (starPEG)-GAG hydrogel variants upon subcutaneous implantation in immunocompetent C57BL/6J mice for up to 28 days. Specifically, we investigated the immune ...
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