Anaerobic bacteria

Treponema denticola

Honeysuckle

Bacteria

Biology

Fusobacterium nucleatum

Eikenella corrodens

Traditional Chinese medicine

Porphyromonas gingivalis

Alternative medicine

Genetics

Antibiotics

Microbiology

Aggressive periodontitis

Internal medicine

Amoxicillin

Periodontitis

Aggregatibacter actinomycetemcomitans

Medicine

Prevotella intermedia

Tannerella forsythia

Pathology

Journal Of Clinical Periodontology

Objective This retrospective surveillance study aimed to follow periodontitis‐associated bacterial profiles and to identify time‐dependent changes in antibiotic susceptibility patterns. Materials and Methods From 2008 to 2015, bacterial specimen from deep periodontal pockets were collected from a total of 7804 German adults diagnosed with periodontitis. Presence of selected bacteria was confirmed by anaerobic culture and nucleic acid amplification. Antimicrobial susceptibility of clinical isolates was tested by disc diffusion with antibiotics used for the treatment of periodontitis and oral infections. The prevalences of periodontal pathogens were calculated and temporal evolution of antimicrobial susceptibility towards amoxicillin, amoxicillin/clavulanic acid, metronidazole, doxycycline, clindamycin, azithromycin, ciprofloxacin and ampicillin was analysed with logistic regression. Results The prevalence of patients harbouring bacteria was 95.9% Fusobacterium nucleatum , 88.0% Tannerella forsythia , 76.4% Treponema denticola , 76.5%, Campylobacter rectus , 76.0% Eikenella corrodens , 75.0% Capnocytophaga spp., 68.2% Porphyromonas gingivalis , 57.7% Peptostreptococcus micros , 43.1% Prevotella intermedia , 30.4% Eubacterium nodatum and 21.5% Aggregatibacter actinomycetemcomitans . In 63.5% of patients, one or more isolates were not susceptible to at least one of the antibiotics tested. The data further revealed a trend towards decreasing susceptibility profiles ( p &lt; 0.05) with antibiotic non‐susceptibilities in 37% of patients in 2008 and in 70% in 2015. Conclusions The present study confirmed a high prevalence of periodontal pathogens in the subgingival microbiota of German periodontitis patients. The data revealed an incremental increase in isolates displaying resistance to some antibiotics but no relevant change in susceptibility to amoxicillin and metronidazole.

Prevalence and antibiotic susceptibility trends of periodontal pathogens in the subgingival microbiota of German periodontitis patients: A retrospective surveillance study