Self-assembled chitosan polymer intercalating peptide functionalized gold nanoparticles as nanoprobe for efficient imaging of urokinase plasminogen activator receptor in cancer diagnostics

Published on Apr 30, 2021in Carbohydrate Polymers7.182
· DOI :10.1016/J.CARBPOL.2021.118138
Deepshikha Shahdeo4
Estimated H-index: 4
,
Veerbhan Kesarwani1
Estimated H-index: 1
+ 3 AuthorsSonu Gandhi19
Estimated H-index: 19
Source
Abstract
Abstract Targeting cell surface receptors for specific drug delivery in cancer has garnered lot of attention. Urokinase plasminogen activator receptor (uPAR), a surface biomarker, is overexpressed on many tumours including breast, colorectal, prostate, and ovarian cancers. Binding of growth factor domain (GFD) of urokinase plasminogen activator (uPA) with uPAR lead to its close conformation, and allow somatomedin B domain (SMB) of vitronectin binding by allosteric modulation. In-silico docking of uPAR with GFD and SMB peptides were performed to identify potential binding affinity. Herein, we report fluorescently labeled peptide functionalized AuNPs with a mixed self-assembled monolayer of intercalating chitosan polymer for efficient targeting and imaging of uPAR-positive cells. The biophysical characterization of nanoconjugates and uPAR-specific targeting was assessed by FACS, cell adhesion, and fluorescence imaging. AuNPs/chitosan/GFD + SMB peptides showed higher uptake as compared to AuNPs/chitosan/GFD, and AuNPs/chitosan/SMB that can be utilized as a tool for molecular targeting and imaging in metastasis.
References62
Newest
#1Aruna Kasoju (Jawaharlal Nehru Technological University, Anantapur)H-Index: 2
#2Deepshikha ShahdeoH-Index: 4
Last. Sonu GandhiH-Index: 19
view all 9 authors...
1 CitationsSource
#1Aruna Kasoju (Jawaharlal Nehru Technological University, Anantapur)H-Index: 2
#2Deepshikha ShahdeoH-Index: 4
Last. Sonu GandhiH-Index: 19
view all 9 authors...
This study describes the colorimetric detection of aflatoxin M1 (Afl M1) in milk samples using a microfluidic paper-based analytical device (µPAD). Fabrication of µPADs was done using a simple and quick approach. Each μPAD contained a detection zone and a sample zone interconnected by microchannels. The colorimetric assay was developed using unmodified AuNPs as a probe and 21-mer aptamer as a recognition molecule. The free aptamers were adsorbed onto the surface of AuNPs in absence of Afl M1, ev...
11 CitationsSource
#1Neeraj Kumar (DU: University of Delhi)H-Index: 1
#1Neeraj Kumar (DU: University of Delhi)H-Index: 14
Last. Ramesh Chandra (DU: University of Delhi)H-Index: 61
view all 4 authors...
The mankind relies on the use of antibiotics for a healthy life. The epidemic-like emergence of drug-resistant bacterial strains is increasingly becoming one of the leading causes of morbidity and mortality, which gives rise to design a potential antimicrobial peptide (AMP). Here, we have designed the potential AMP using the extensive dynamics simulation since protein–peptide interactions are linked to large conformational changes. Therefore, we have employed the advanced computational avenue CA...
4 CitationsSource
#1Akanksha RobertsH-Index: 3
#2Prem Prakash Tripathi (IICB: Indian Institute of Chemical Biology)H-Index: 12
Last. Sonu GandhiH-Index: 19
view all 3 authors...
Abstract Fluorine doped tin oxide (FTO) electrochemical immunosensor has been developed for rapid detection of urokinase type plasminogen activator receptor (uPAR) - a biomarker for cancer. uPAR is a GPI-anchored cell membrane receptor that shows increased expression in many types of human cancers which include breast, prostate, colorectal, and non-small cell lung cancer. In this study, a novel ultrasensitive FTO graphene nanosheets based electrode was used as a working probe to analyze the inte...
13 CitationsSource
#1Nathalia Rodrigues de Almeida (UNMC: University of Nebraska Medical Center)H-Index: 6
#2Yuchun Han (CAS: Chinese Academy of Sciences)H-Index: 19
Last. Martin Conda Sheridan (UNMC: University of Nebraska Medical Center)H-Index: 1
view all 6 authors...
The development of bacterial resistant strains is a global health concern. Designing antibiotics that limit the rise of pathogenic resistance is essential to efficiently treat pathogenic infections. Self-assembling amphiphilic molecules are an intriguing platform for the treatment of pathogens because of their ability to disrupt bacterial membranes and function as drug nanocarriers. We have designed cationic peptide amphiphiles (PAs) that can form micelles, nanofibers, and twisted ribbons with t...
23 CitationsSource
#1Prem Prakash Tripathi (IICB: Indian Institute of Chemical Biology)H-Index: 12
#2Hamed Arami (Stanford University)H-Index: 30
Last. Sonu GandhiH-Index: 19
view all 5 authors...
// Prem Prakash Tripathi 1, 2 , Hamed Arami 3, 4 , Ivneet Banga 5 , Jalaj Gupta 6 and Sonu Gandhi 7 1 CSIR-Indian Institute of Chemical Biology (CSIR-IICB), Kolkata, India 2 IICB-Translational Research Unit of Excellence, Kolkata, India 3 Molecular Imaging Program at Stanford (MIPS), The James H. Clark Center, Stanford University, Stanford, CA, USA 4 Department of Radiology, Stanford University, School of Medicine, Stanford, CA, USA 5 Department of Bioengineering, University of Texas, Arlington,...
31 CitationsSource
#1Anita Talan (Amity Institute of Biotechnology)H-Index: 1
#2Annu Mishra (Amity University)H-Index: 4
Last. Sonu Gandhi (Amity Institute of Biotechnology)H-Index: 19
view all 6 authors...
Abstract Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly toxic for human health. Fluorine doped tin-oxide (FTO) based electrochemical nanosensor was developed for chlorpyrifos detection with gold nanoparticles (AuNPs) and anti-chlorpyrifos antibodies (chl-Ab). AuNPs provides high electrical conductivity and specific resistivity, thus increases the sensitivity of immunoassay. High electrical conductivity of AuNPs reveals that it promotes the redox rea...
51 CitationsSource
#1Kalishwaralal Kalimuthu (Ariel University)H-Index: 4
#2Bat-Chen Lubin (Ariel University)H-Index: 1
Last. Michael A. Firer (Ariel University)H-Index: 23
view all 7 authors...
Background Peptide-drug-conjugates (PDCs) are being developed as an effective strategy to specifically deliver cytotoxic drugs to cancer cells. However one of the challenges to their successful application is the relatively low stability of peptides in the blood, liver and kidneys. Since AuNPs seem to have a longer plasma half-life than PDCs, one approach to overcoming this problem would be to conjugate the PDCs to gold nanoparticles (AuNPs), as these have demonstrated favorable physico-chemical...
32 CitationsSource
#1Sonu Gandhi (Amity University)H-Index: 19
#2Ivneet Banga (Amity University)H-Index: 5
Last. Sergei A. Eremin (MSU: Moscow State University)H-Index: 41
view all 4 authors...
Gold nanoparticle (AuNP)-based optical assays are of significant interest since the molecular phenomenon can be examined easily with change in the color of AuNPs. Herein, we report the development of a dipstick using a AuNP-labeled single-chain fragment variable (scFv) antibody for the detection of morphine. The scFv antibodies for morphine were developed using phage display-based antibody library. Immunoglobulin variable regions of heavy (VH)- and light (VL)-chain genes were connected via a gly...
17 CitationsSource
#1Michela Romanini (UPC: Polytechnic University of Catalonia)H-Index: 12
#2Maria Barrio (UPC: Polytechnic University of Catalonia)H-Index: 31
Last. Josep Ll. Tamarit (UPC: Polytechnic University of Catalonia)H-Index: 16
view all 6 authors...
We probe the temperature- and pressure-dependent specific volume (v) and dipolar dynamics of the amorphous phase (in both the supercooled liquid and glass states) of the ternidazole drug (TDZ). Three molecular dynamic processes are identified by means of dielectric spectroscopy, namely the a relaxation, which vitrifies at the glass transition, a Johari-Goldstein sJG relaxation, and an intramolecular process associated with the relaxation motion of the propanol chain of the TDZ molecule. The line...
51 CitationsSource
Cited By0
Newest