The bilayer skin substitute based on human adipose-derived mesenchymal stem cells and neonate keratinocytes on the 3D nanofibrous PCL-platelet gel scaffold

In the present study, neonate keratinocytes were cocultured with human adipose-derived mesenchymal stem cells (hAMSCs) on the electrospun polycaprolactone-platelet gel (PCL–PG) scaffold. To evaluate its potential of wound healing and skin tissue engineering, neonate keratinocytes must be differentiated. The PCL scaffold prepared by the electrospinning technique was fabricated and coated by PG. Then scaffolds were fully characterized by SEM,...