Effects of Substituting Arginine by Lysine in Bovine Lactoferricin Derived Peptides: Pursuing Production Lower Costs, Lower Hemolysis, and Sustained Antimicrobial Activity

Monomeric, dimeric, palindromic, and tetrameric peptides derived from bovine lactoferricin (LfcinB) containing the RRWQWR or the KKWQWK sequence were synthesized via SPPS- Fmoc/tBu strategy, and Lys-peptides showed higher synthesis efficiency. The antibacterial activity of the peptides against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC reference strains and a multidrug resistant clinical isolate was evaluated. Lys...