An efficient high‐speed countercurrent chromatography method for preparative isolation of highly potent anti‐cancer compound antroquinonol from Antrodia camphorata after experimental design optimized extraction

To avoid irreversible stationary phase adsorption and tedious and time-consuming separation steps, high-speed countercurrent chromatography was employed for the preparative separation of anti-tumor compound antroquinonol from solid fermentation culture of Antrodia camphorata for the first time. A Box-Behnken experimental design, based on three parameters including liquid-to-solid ratio, extraction time, and extraction temperature, was applied to...