A CRISPR/Cas12a-assisted platform for identification and quantification of single CpG methylation sites

Published: Apr 6, 2021
Abstract
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/associated nuclease (Cas) systems have repeatedly shown to have excellent performance in nucleotide sensing applications1–5. High specificity and selectivity of Cas effector proteins is determined by the CRISPR RNA’s (crRNA’s) interchangeable spacer sequence, as well as position and number of mismatches between target sequence and the crRNA sequence1. Some diseases are...
Paper Details
Title
A CRISPR/Cas12a-assisted platform for identification and quantification of single CpG methylation sites
Published Date
Apr 6, 2021
Journal
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