New methodology for separating lignin fractions: GPC on a preparative scale with stationary phase of hydrogel derived from cellulose acetate

In this paper a new stationary phase was studied, a hydrogel derived from cellulose acetate (DS 2.5) and ethylenediaminetetraacetic dianhydride (HEDTA) was used in gel permeation chromatography (GPC) on a preparative scale for elution of acetosolv lignin samples. Initially, tests were performed to verify the potential of HEDTA as a stationary phase for separation of lignin fragments and sugars, using sodium phenolate to simulate lignin, and...