A Novel Graphene Quantum Dot-Based mRNA Delivery Platform.

Published on Apr 7, 2021in ChemistryOpen2.37
· DOI :10.1002/OPEN.202000200
Ya Liu4
Estimated H-index: 4
(Fudan University),
Changhong Zhao5
Estimated H-index: 5
(Chalmers University of Technology)
+ 4 AuthorsJohan Liu54
Estimated H-index: 54
(Chalmers University of Technology)
During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh-7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first-generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.
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