Icariside II promotes the differentiation of human amniotic mesenchymal stem cells into dopaminergic neuron-like cells.

Published on Mar 15, 2021in In Vitro Cellular & Developmental Biology – Animal1.665
· DOI :10.1007/S11626-021-00556-8
Kuang Wei1
Estimated H-index: 1
(Zunyi Medical College),
Tao Liu2
Estimated H-index: 2
(Zunyi Medical College)
+ 3 AuthorsChangyin Yu3
Estimated H-index: 3
(Zunyi Medical College)
Sources
Abstract
The purpose of this study is to observe the effect of icariside II (ICS II) on the differentiation of human amniotic mesenchymal stem cells (hAMSCs) into dopaminergic neuron-like cells, the involvement of PI3K signaling pathway inhibitors. After identifying hAMSCs by flow cytometry, hAMSCs were induced and treated with ICS II at 10 μmol/L, 3 μmol/L, 1 μmol/L, and 0 μmol/L. hAMSCs in the LY294002+3μM ICS II group were pretreated with 20 μmol/L LY294002, a PI3K-specific inhibitor, for 1 h, and then hAMSCs were induced with 3 μmol/L ICS II. On the 21st day of induction, immunofluorescence was used to detect expression of the neuronal nuclei (NeuN), neuron-specific enolase (NSE), microtubule-associated protein-2 (MAP-2), glial fibrillary acidic protein (GFAP), and tyrosine hydroxylase (TH) antigens in each induced cell group. Western blotting was used to detect the relative protein expression of NSE, MAP-2, GFAP, and TH. ELISA was used to detect the dopamine concentration in the induction medium supernatant of each group. After 21 d of ICS II induction, immunofluorescence showed that GFAP expression was not obvious in any hAMSC group. The NeuN, NSE, MAP-2, and TH fluorescent proteins were expressed in each group. NeuN was expressed in the nucleus and cytoplasm, while NSE, MAP-2, and TH were mainly expressed in the cytoplasm. The positive cell rates of NeuN, NSE, MAP-2, and TH in the 10 μmol/L, 3 μmol/L, and 1 μmol/L ICS II groups were higher than those in the LY294002+3μM ICS II and control groups. After 21 d of induction, the Western blot results showed that the protein expression levels of NSE, MAP-2, and TH in the 10 μmol/L, 3 μmol/L, and 1 μmol/L ICS II groups were significantly higher than those in the LY294002+3μM ICS II and control groups. The MAP-2 protein expression levels in the 10 μmol/L and 3 μmol/L groups were higher than that in the 1 μmol/L group. After 21 d of induction, the dopamine concentrations in the culture supernatants of the 10 μmol/L, 3 μmol/L, and 1 μmol/L ICS II groups were higher than those in the LY294002+3μM ICS II and control groups. In our experiment, ICS II induced hAMSCs to differentiate into dopaminergic neuron-like cells, and the optimal concentration range of ICS II was 3–10 μmol/L. Moreover, the PI3K signaling pathway is involved in the above differentiation process.
📖 Papers frequently viewed together
References31
Newest
#1Yanjun Zhang (CAS: Chinese Academy of Sciences)H-Index: 8
#2Ruoqi Huang (CAS: Chinese Academy of Sciences)H-Index: 3
Last. Qiong Liang (CAS: Chinese Academy of Sciences)H-Index: 2
view all 5 authors...
AbstractEpimedium brevicornu Maxim. is a traditional Chinese medicine herb with good effects on many diseases. In the present paper, the complete chloroplast genome of E. brevicornu was sequenced. ...
4 CitationsSource
#1Fang HeH-Index: 1
#2Wang YuyingH-Index: 3
Last. Limei YuH-Index: 11
view all 4 authors...
Abstract Aims To investigate the therapeutic effects of human amniotic mesenchymal stem cells (hAMSCs) on paraquat (PQ)-induced pulmonary fibrosis in rats and investigate the inflammatory mechanisms. Main methods hAMSCs were identified by morphological, flow cytometry and immunocytochemistry. A pulmonary fibrosis model was induced by administering PQ to rats. The hAMSC group was treated with hAMSCs after 6 h of PQ poisoning. At 21 days after hAMSC transplantation, lungs were harvested for H&E, M...
6 CitationsSource
#1Melissa J. Armstrong (UF: University of Florida)H-Index: 24
#2Michael S. Okun (UF: University of Florida)H-Index: 73
Importance Parkinson disease is the most common form of parkinsonism, a group of neurological disorders with Parkinson disease–like movement problems such as rigidity, slowness, and tremor. More than 6 million individuals worldwide have Parkinson disease. Observations Diagnosis of Parkinson disease is based on history and examination. History can include prodromal features (eg, rapid eye movement sleep behavior disorder, hyposmia, constipation), characteristic movement difficulty (eg, tremor, st...
138 CitationsSource
#1Malin Parmar (Lund University)H-Index: 37
#2Shane Grealish (Lund University)H-Index: 17
Last. Claire Henchcliffe (Cornell University)H-Index: 29
view all 3 authors...
Cell-replacement therapies have long been an attractive prospect for treating Parkinson disease. However, the outcomes of fetal tissue-derived cell transplants in individuals with Parkinson disease have been variable, in part owing to the limitations of fetal tissue as a cell source, relating to its availability and the lack of possibility for standardization and to variation in methods. Advances in developmental and stem cell biology have allowed the development of cell-replacement therapies th...
39 CitationsSource
#1Chunyang HeH-Index: 2
#2Ze WangH-Index: 2
Last. Jing-Shan Shi (Zunyi Medical College)H-Index: 31
view all 3 authors...
Abstract Icariin (ICA) is a principal active component from traditional Chinese medicine Epimedium grandiflorum. To explain its traditional medical usages by modern science, a variety of pharmacological effects have been studied for ICA. In this review, we summarized the pharmacokinetics of ICA as well as its pharmacological mechanisms in neurodegenerative disease, cardiovascular disease, anti-osteoporosis, anti-inflammation, anti-oxidative stress, anti-depression and anti-tumors.
8 CitationsSource
#1Suresh B. Rangasamy (Rush University Medical Center)H-Index: 10
#2Sridevi Dasarathi (Rush University Medical Center)H-Index: 5
Last. Kalipada Pahan (Rush University Medical Center)H-Index: 55
view all 5 authors...
Increasing the function of residual dopaminergic neurons in the nigra of PD patients is an important area of research as it may eventually compensate the loss. Although tyrosine hydroxylase (TH) is the rate-limiting enzyme in the dopamine (DA) biosynthesis pathway, there are no effective drugs/molecules to upregulate TH and increase the production of DA in nigral dopaminergic neurons. This study underlines the importance of aspirin in stimulating the expression of TH and increasing the level of ...
13 CitationsSource
#1Tao Liu (Zunyi Medical College)H-Index: 2
#2Fang He (Zunyi Medical College)H-Index: 2
Last. Changyin Yu (Zunyi Medical College)H-Index: 3
view all 5 authors...
Chronic cerebral hypoperfusion (CCH) is a basic pathological process that is comorbid with brain diseases, such as vascular Parkinsonism and Alzheimer’s disease. Icariside II (ICS II), which is one of the main metabolites of icariin, has anti-inflammatory and antioxidant effects and protects against ischemic brain injury. This study aims to investigate the neuroprotective effects of ICS II on neuronal axon regeneration-related factors in a CCH rat model. Sprague-Dawley (SD) rats were divided int...
5 Citations
#1Linda A. Hershey (University of Oklahoma Health Sciences Center)H-Index: 12
#2Rhonda Coleman-Jackson (University of Oklahoma Health Sciences Center)H-Index: 1
Dementia with Lewy bodies (DLB) is a complex disease that involves a variety of cognitive, behavioral and neurological symptoms, including progressive memory loss, visual hallucinations, parkinsonism, cognitive fluctuations and rapid eye movement sleep behavior disorder (RBD). These symptoms may appear in varying combinations and levels of severity in each patient who is seen in the clinic, making diagnosis and treatment a challenge. DLB is the third most common of all the neurodegenerative dise...
26 CitationsSource
#1Raffaella Lazzarini (Marche Polytechnic University)H-Index: 20
#2Simone Guarnieri (University of Chieti-Pescara)H-Index: 20
Last. Roberto Di Primio (Marche Polytechnic University)H-Index: 19
view all 9 authors...
Mesenchymal stem cells (MSCs) are well-characterized adult stem cells, recently isolated from human nucleus pulposus of degenerate and non-degenerate intervertebral disc. The attention to this source is linked to its embryologic history and cells may conserve a stronger aptitude to neuronal differentiation than other MSCs. Here, MSCs from nucleus pulposus (NP-MSCs) were successfully isolated and characterized for morphology, proliferation, and expression of selected genes. Subsequently, the neur...
6 CitationsSource
#1Eunhye Kim (CBNU: Chungbuk National University)H-Index: 9
#2Seon Ung Hwang (CBNU: Chungbuk National University)H-Index: 5
Last. Sang-Hwan Hyun (CBNU: Chungbuk National University)H-Index: 25
view all 6 authors...
Background The porcine brain is gyrencephalic with similar gray and white matter composition and size more comparable to the human rather than the rodent brain; however, there is lack of information about neural progenitor cells derived from this model.
1 CitationsSource
Cited By0
Newest