Characterization of xylitol 4-dehydrogenase from Erwinia aphidicola and its co-expression with NADH oxidase in Bacillus subtilis
Abstract
L-Xylulose is a rare ketose with potential applications in the pharmaceutical and food industries. This metabolic intermediate can be synthesized by xylitol 4-dehydrogenase (XDH) using xylitol as substrate and NAD+ as a cofactor. A novel XDH from Erwinia aphidicola SK47.001 was expressed in food-grade Bacillus subtilis and biochemically characterized. The purified enzyme exhibited an optimal temperature of 45 °C, an optimal pH of 11, a Km of...
Paper Details
Title
Characterization of xylitol 4-dehydrogenase from Erwinia aphidicola and its co-expression with NADH oxidase in Bacillus subtilis
Published Date
May 1, 2021
Journal
Volume
104
Pages
92 - 100
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