Gamma radiation-induced grafting of poly(2-aminoethyl methacrylate) onto chitosan: A comprehensive study of a polyurethane scaffold intended for skin tissue engineering.

Published on Oct 15, 2021in Carbohydrate Polymers7.182
· DOI :10.1016/J.CARBPOL.2021.117916
Maykel González-Torres6
Estimated H-index: 6
,
Ilian Haide Serrano-Aguilar (UNAM: National Autonomous University of Mexico)+ 9 AuthorsCristina Velasquillo10
Estimated H-index: 10
Source
Abstract
Abstract A novel brush-like poly(2-aminoethyl methacrylate) (PAEMA) was grafted onto chitosan (CS) through gamma radiation-induced polymerization. The copolymer (CS-g-PAEMA) was used to prepare a sodium acetate leached poly(urethane-urea) scaffold. The above derivatives were developed, synthesized, and characterized to meet the specific characteristics of biomaterials. The results revealed that this method is an easy and successful route for grafting PAEMA onto CS. The feasibility of preparing a CS-g-PAEMA polyurethane foam was confirmed by mechanical, morphometric, spectroscopic, and cytotoxic studies. The scaffold showed high biocompatibility both in vitro and in vivo. The first experiment proved that CS-based polyurethane efficiently allows the dynamic culturing of human fibroblast cells. Additionally, an in vivo study in a murine model indicated a complete integration of the scaffold to surrounding subcutaneous tissue as supported by the histological and histochemical assessments. The aforementioned results support the use of CS-g-PAEMA poly(saccharide-urethane) as a model of in vitro-engineered skin.
References66
Newest
#1Sita ShresthaH-Index: 9
Last. Cheol Sang KimH-Index: 51
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Abstract A biomimetic-based approaches, especially with artificial scaffolding, have established great potential to provide tissue regeneration capacity and an effective way to bridge the gap between host cell responses and organ demands. However, the synthesis of biomaterial is most efficient when the functional behavior involved most resembles the natural extracellular matrix. Here, a fibrous scaffold was engineered by integrating zein and chitosan (CS) in to polyurethane (PU) associated with ...
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#2Danilo Martins dos Santos (Empresa Brasileira de Pesquisa Agropecuária)H-Index: 12
Last. Luiz Alberto Colnago (Empresa Brasileira de Pesquisa Agropecuária)H-Index: 32
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ABSTRACT We propose a novel approach relied on high-resolution solid-state 13C NMR spectroscopy to quantify the crystallinity index of chitosans (Ch) prepared with variable average degrees of acetylation ( D A ¯ ) from 5% to 60% and average weight molecular weight ( M ¯ w ) ranged in 0.15 × 106 g mol-1-1.2 × 106 g mol-1. The Dipolar Chemical Shift Correlation (DIPSHIFT) curve of the C(6)OH segment revealed increased mobility dynamic, which induced different distribution from trans-to-gauche conf...
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Last. Rogelio Rodríguez-Talavera (UNAM: National Autonomous University of Mexico)H-Index: 2
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Abstract This study aimed at investigating the synthesis, characterization, and search for a biotechnological application proposal for poly [(R)-3-hydroxybutyric acid] (PHB) grafted with the n-hydroxyethyl acrylamide (HEAA) monomer. The novel copolymer was prepared by 60Co gamma radiation-induced-graft polymerization. The effect of different solvents in the graft polymerization; the degree of grafting, crystallinity, and hydrophilicity; the morphology and the thermal properties were evaluated. T...
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#1Nisar AliH-Index: 17
#1Nisar AliH-Index: 14
Last. Hafiz M.N. Iqbal (Tec: Monterrey Institute of Technology and Higher Education)H-Index: 63
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Abstract Herein, we report the development and characterization of a green sorbent material. For the said purpose, the chitosan’s capacity of cation sorption was enhanced by incorporating thiocarabamate group, which ultimately increases active sites for attracting the cationic species. The process was carried out by utilizing acrylomorpholine, carbon disulfide and chitosan in a single stage process. The product (CH-ATC) acquired was well characterized with 13C NMR (solid-form), FTIR spectroscopy...
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#2Abbas Mohammadi (UI: University of Isfahan)H-Index: 11
Last. Anousheh Zargar Kharazi (IUMS: Isfahan University of Medical Sciences)H-Index: 7
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Abstract In this study, a polyurethane nanocomposite film (PU/CS-GO) was synthesized by incorporating chitosan-modified GO nanosheets (CS-GO) into PU matrices. The successful synthesis of unmodified GO and CS-GO nanosheets was confirmed by FTIR, XRD, Raman, SEM, EDS, and TGA analyses. In comparison with polyurethane nanocomposite containing unmodified graphene oxide (PU/GO), the effect of the modification of GO with chitosan on different properties such as morphological, thermal, thermo-mechanic...
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