High-content, high-throughput imaging of single-cell metabolism by sub-sampled hyperspectral SRS microscopy

Hyperspectral stimulated Raman scattering (hSRS) is a label-free microspectroscopic modality that enables live-cell metabolic imaging with chemical specificity. Yet, hSRS in the CH region has low throughput and poor chemical specificity, which limits its application to a broader range of metabolic studies. We propose a high-content, high-throughput hSRS imaging method by a sparsity-driven spectral unmixing and active spectral sub-sampling. We...