Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

Volume: 2, Issue: 1, Pages: 100318 - 100318
Published: Mar 1, 2021
Abstract
Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol...
Paper Details
Title
Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
Published Date
Mar 1, 2021
Volume
2
Issue
1
Pages
100318 - 100318
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