Non-genetic acoustic stimulation of single neurons by a tapered fiber optoacoustic emitter

Published on Dec 17, 2020in arXiv: Neurons and Cognition
Linli Shi1
Estimated H-index: 1
(BU: Boston University),
Ying Jiang4
Estimated H-index: 4
(BU: Boston University)
+ 6 AuthorsChen Yang74
Estimated H-index: 74
(BU: Boston University)
As an emerging technology, transcranial focused ultrasound has been demonstrated to successfully evoke motor responses in mice, rabbits, and sensory/motor responses in humans. Yet, the spatial resolution of ultrasound does not allow for high-precision stimulation. Here, we developed a tapered fiber optoacoustic emitter (TFOE) for optoacoustic stimulation of neurons with an unprecedented spatial resolution of 20 microns, enabling selective activation of single neurons or subcellular structures, such as axons and dendrites. A single acoustic pulse of 1 microsecond converted by the TFOE from a single laser pulse of 3 nanoseconds is shown as the shortest acoustic stimuli so far for successful neuron activation. The highly localized ultrasound generated by the TFOE made it possible to integrate the optoacoustic stimulation and highly stable patch clamp recording on single neurons. Direct measurements of electrical response of single neurons to acoustic stimulation, which is difficult for conventional ultrasound stimulation, have been demonstrated for the first time. By coupling TFOE with ex vivo brain slice electrophysiology, we unveil cell-type-specific response of excitatory and inhibitory neurons to acoustic stimulation. These results demonstrate that TFOE is a non-genetic single-cell and sub-cellular modulation technology, which could shed new insights into the mechanism of neurostimulation.
#1Linli Shi (BU: Boston University)H-Index: 1
#2Ying Jiang (BU: Boston University)H-Index: 4
Last. Chen Yang (BU: Boston University)H-Index: 74
view all 7 authors...
Abstract Focused ultrasound has attracted great attention in minimally invasive therapeutic and mechanism studies. Frequency below 1 MHz is identified preferable for high-efficiency bio-modulation. However, the poor spatial confinement of several millimeters and large device diameter of ∼25 mm of typical sub-MHz ultrasound technology suffered from the diffraction limit, severely hindering its further applications. To address it, a fiber-based optoacoustic emitter (FOE) is developed, serving as a...
#1Ying Jiang (BU: Boston University)H-Index: 4
#2Hyeon Jeong LeeH-Index: 11
Last. Ji-Xin ChengH-Index: 84
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Low-intensity ultrasound is an emerging modality for neuromodulation. Yet, transcranial neuromodulation using low-frequency piezo-based transducers offers poor spatial confinement of excitation volume, often bigger than a few millimeters in diameter. In addition, the bulky size limits their implementation in a wearable setting and prevents integration with other experimental modalities. Here, we report spatially confined optoacoustic neural stimulation through a miniaturized Fiber-Optoacoustic C...
#1Augix Guohua Xu (ZJU: Zhejiang University)H-Index: 4
#2Meizhen Qian (ZJU: Zhejiang University)H-Index: 3
Last. Anna W. RoeH-Index: 41
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We have developed a way to map brain-wide networks using focal pulsed infrared neural stimulation in ultrahigh-field magnetic resonance imaging (MRI). The patterns of connections revealed are similar to those of connections previously mapped with anatomical tract tracing methods. These include connections between cortex and subcortical locations and long-range cortico-cortical connections. Studies of local cortical connections reveal columnar-sized laminar activation, consistent with feed-forwar...
#1Christian Ineichen (UZH: University of Zurich)H-Index: 9
Last. Oǧuzkan SürücüH-Index: 1
view all 3 authors...
Deep brain stimulation (DBS) has been proven to be an effective treatment modality for various late-stage neurological and psychiatric disorders. However, knowledge on the electrical field distribution in the brain tissue is still scarce. Uptodate,Most recent attempts to e perspective of understand ding electric field spread was were primarily focused on the effect of different electrodes on rather simple tissue models. The influence of microanatomic, biophysical tissue properties in particular ...
#1Hod Dana (HHMI: Howard Hughes Medical Institute)H-Index: 17
#2Yi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 15
Last. Douglas S. Kim (HHMI: Howard Hughes Medical Institute)H-Index: 24
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Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal cell bodies, or to follow dynamics in subcellular compartments such as axons, dendrites and individual synaptic compartments. Despite major advances, calcium imaging is still limited by the biophysical properties of existing GECIs, including affinity, ...
#1Fernando R. Fernandez (BU: Boston University)H-Index: 19
#2Bahar Rahsepar (BU: Boston University)H-Index: 3
Last. John A. White (BU: Boston University)H-Index: 40
view all 3 authors...
Abstract Synaptic activity in vivo can potentially alter the integration properties of neurons. Using recordings in awake mice, we targeted somatosensory layer 2/3 pyramidal neurons and compared neuronal properties with those from slices. Pyramidal cells in vivo had lower resistance and gain values, as well as broader spikes and increased spike frequency adaptation compared to the same cells in slices. Increasing conductance in neurons using dynamic clamp to levels observed in vivo , however, di...
#1Jan Kubanek (Stanford University)H-Index: 13
The understanding of brain function and the capacity to treat neurological and psychiatric disorders rest on the ability to intervene in neuronal activity in specific brain circuits. Current methods of neuromodulation incur a tradeoff between spatial focus and the level of invasiveness. Transcranial focused ultrasound (FUS) is emerging as a neuromodulation approach that combines noninvasiveness with focus that can be relatively sharp even in regions deep in the brain. This may enable studies of ...
#1Sacha Noimark (UCL: University College London)H-Index: 20
#2Richard J. Colchester (UCL: University College London)H-Index: 10
Last. Adrien E. Desjardins (UCL: University College London)H-Index: 31
view all 18 authors...
Polydimethylsiloxane (PDMS) is widely used in biomedical science and can form composites that have broad applicability. One promising application where PDMS composites offer several advantages is optical ultrasound generation via the photoacoustic effect. Here, methods to create these PDMS composites are reviewed and classified. It is highlighted how the composites can be applied to a range of substrates, from micrometer‐scale, temperature‐sensitive optical fibers to centimeter‐scale curved and ...
#1Or A. ShemeshH-Index: 11
#2Dimitrii Tanese (Paris V: Paris Descartes University)H-Index: 16
Last. Valentina Emiliani (Paris V: Paris Descartes University)H-Index: 31
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Optogenetic control of individual neurons with high temporal precision within intact mammalian brain circuitry would enable powerful explorations of how neural circuits operate. Two-photon computer-generated holography enables precise sculpting of light and could in principle enable simultaneous illumination of many neurons in a network, with the requisite temporal precision to simulate accurate neural codes. We designed a high-efficacy soma-targeted opsin, finding that fusing the N-terminal 150...
#1Go Eun Ha (Yonsei University)H-Index: 6
#2Eunji Cheong (Yonsei University)H-Index: 25
: Neuronal firing patterns and frequencies determine the nature of encoded information of the neurons. Here we discuss the molecular identity and cellular mechanisms of spike-frequency adaptation in central nervous system (CNS) neurons. Calcium-activated potassium (KCa) channels such as BKCa and SKCa channels have long been known to be important mediators of spike adaptation via generation of a large afterhyperpolarization when neurons are hyper-activated. However, it has been shown that a stron...
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