Search for SARS-CoV-2 RNA in platelets from COVID-19 patients.

Published on Feb 17, 2021in Platelets3.378
· DOI :10.1080/09537104.2020.1859104
Loredana Bury16
Estimated H-index: 16
(University of Perugia),
Barbara Camilloni12
Estimated H-index: 12
(University of Perugia)
+ 17 AuthorsPaolo Gresele69
Estimated H-index: 69
(University of Perugia)
Source
Abstract
The frequent finding of thrombocytopenia in patients with severe SARS-CoV-2 infection (COVID-19) and previous evidence that several viruses enter platelets suggest that SARS-CoV-2 might be internalized by platelets of COVID-19. Aim of our study was to assess the presence of SARS-CoV-2 RNA in platelets from hospitalized patients with aconfirmed diagnosis of COVID-19. RNA was extracted from platelets, leukocytes and serum from 24 COVID-19 patients and 3 healthy controls, real-time PCR and ddPCR for viral genes were carried out. SARS-CoV-2 RNA was not detected in any of the samples analyzed nor in healthy controls, by either RT-PCR or ddPCR, while RNA samples from nasopharyngeal swabs of COVID-19 patients were correctly identified. Viral RNA was not detected independently of viral load, of positive nasopharyngeal swabs, or viremia, the last detected in only one patient (4.1%). SARS-CoV-2 entry in platelets is not acommon phenomenon in COVID-19 patients, differently from other viral infections.
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