Transcriptome analysis of pig macrophages expressing porcine reproductive and respiratory syndrome virus non-structural protein 1

Published on Jan 1, 2021in Veterinary Immunology and Immunopathology2.046
· DOI :10.1016/J.VETIMM.2020.110147
In Byung Park1
Estimated H-index: 1
(KU: Korea University),
Yong Chan Choi1
Estimated H-index: 1
(KU: Korea University)
+ 1 AuthorsTaehoon Chun22
Estimated H-index: 22
(KU: Korea University)
Sources
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative pathogen of PRRS, one of the most economically disastrous swine diseases. Non-structural protein 1 (NSP1) of PRRSV consists of NSP1α and NSP1β which exhibit papain like cysteine protease activity. Recent evidence demonstrates that PRRSV NSP1 may be participated in modulating host immunity, but very few host proteins were discovered as targets for NSP1. In this study, we used RNA-seq to investigate the functional role of PRRSV NSP1 in porcine alveolar macrophages, 3D4/31 cells. Compared to empty vector (mock) transfectant, NSP1, NSP1α, and NSP1β expressing 3D4/31 cells displayed a total of 60 genes, 63 genes, and 80 genes as differentially expressed genes (DEGs), respectively. Most of DEGs are involved in early inflammatory responses including interleukin (IL)-17 signaling pathway, chemokine signaling pathway, tumor necrosis factor (TNF)-α signaling pathway, and cell adhesion molecules. Interestingly, PRRSV NSP1 expression in 3D4/31 cells decreased mRNA transcripts of Fosb and Gdf15 known to be involved in host cell signaling or host cell protection during inflammation. Therefore, PRRSV NSP1 might block the signaling involved in host immune surveillance. Further study is required to define the mechanism on how PRRSV NSP1 protein represses mRNA transcripts of specific host genes.
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References30
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#1Harding H. Luan (Yale University)H-Index: 10
#2Andrew Wang (Yale University)H-Index: 18
Last. Ruslan Medzhitov (Yale University)H-Index: 123
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Summary Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. We found that inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance. Inde...
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#1Chang Yong Choi (KU: Korea University)H-Index: 6
#2Yong Chan Choi (KU: Korea University)H-Index: 1
Last. Taehoon Chun (KU: Korea University)H-Index: 22
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Abstract Identifying the functional role of each porcine circovirus type 2 (PCV2) ORF associated with host cell modulation might provide better knowledge about the pathogenesis of postweaning multisystemic wasting syndrome (PMWS). PCV2 ORF5 was recently identified and the functional role of ORF5 during pathogenesis after PCV2 infection is largely unknown. In this study, we used RNA-seq to investigate the functional role of PCV2 ORF5 in PCV2-infected porcine epithelial cells. Our data demonstrate...
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#1Nanfang Zeng (CAU: China Agricultural University)H-Index: 3
#2Cong Wang (CAU: China Agricultural University)H-Index: 7
Last. Hanchun Yang (CAU: China Agricultural University)H-Index: 33
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Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens and causes reproductive failure in sows and respiratory disease in growing pigs. PRRSV mainly infects porcine alveolar macrophages (PAMs), leading to the subversion of innate and adaptive immunity of pigs. The transcriptome analysis of gene expression profiles in PRRSV-infected PAMs is essential for understanding the pathogenesis of PRRSV. Here we performed next-generation RNA se...
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#1Taofeng Du (NWAFU: Northwest A&F University)H-Index: 12
#2Yuchen Nan (NWAFU: Northwest A&F University)H-Index: 19
Last. En-Min Zhou (NWAFU: Northwest A&F University)H-Index: 21
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PRRSV (porcine reproductive and respiratory syndrome virus) is a major economically significant pathogen that has adversely impacted the global swine industry for almost 30 years. Currently PRRSV is estimated to cause losses of almost US$600 million per year in the USA. Except for new mutants that continually emerge during PRRSV outbreaks, our understanding of the virology, origin, and evolution of PRRSV and the host's immune response are largely inadequate. Such limited knowledge impedes develo...
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#1Palida Abulizi (UWO: University of Western Ontario)H-Index: 1
#2Neruja Loganathan (UWO: University of Western Ontario)H-Index: 1
Last. Xiufen ZhengH-Index: 30
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Septic acute kidney injury (AKI) and myocardial dysfunction are leading causes of mortality with no accepted method of therapy. In this study we demonstrate the role of growth differentiating factor 15 (GDF15) in septic AKI and myocardial dysfunction using a murine lipopolysaccharide (LPS)-induced sepsis model and an in vitro cell culture system. Data show that GDF15 deficiency augments inflammatory response and exacerbates renal and cardiac injury induced by LPS, while over-expression of GDF15 ...
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#1Hanzhong Ke (UIUC: University of Illinois at Urbana–Champaign)H-Index: 8
#2Dongwan Yoo (UIUC: University of Illinois at Urbana–Champaign)H-Index: 40
Abstract Porcine reproductive and respiratory syndrome (PRRS) remains one of the most economically significant diseases in the swine industry worldwide. The current vaccines are less satisfactory to confer protections from heterologous infections and long-term persistence, and the need for better vaccines are urgent. The immunological hallmarks in PRRSV-infected pigs include the unusually poor production of type I interferons (IFNs-α/β) and the aberrant and delayed adaptive immune responses, ind...
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#1Mihaela Pertea (Johns Hopkins University)H-Index: 38
#2Daehwan Kim (Johns Hopkins University)H-Index: 10
Last. Steven L. SalzbergH-Index: 154
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Pertea et al. describe a protocol to analyze RNA-seq data using HISAT, StringTie and Ballgown (the ‘new Tuxedo’ package). The protocol can be used for assembly of transcripts, quantification of gene expression levels and differential expression analysis.
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#1Joan K. Lunney (USDA: United States Department of Agriculture)H-Index: 32
This review addresses important issues of porcine reproductive and respiratory syndrome virus (PRRSV) infection, immunity, pathogenesis, and control. Worldwide, PRRS is the most economically important infectious disease of pigs. We highlight the latest information on viral genome structure, pathogenic mechanisms, and host immunity, with a special focus on immune factors that modulate PRRSV infections during the acute and chronic/persistent disease phases. We address genetic control of host resis...
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#1Qingzhan Zhang (UIUC: University of Illinois at Urbana–Champaign)H-Index: 9
#2Dongwan Yoo (UIUC: University of Illinois at Urbana–Champaign)H-Index: 40
Abstract Porcine reproductive and respiratory syndrome virus (PRRSV) is endemic in most pig producing countries worldwide and causes enormous economic losses to the swine industry. Specifically differentiated porcine alveolar macrophages are the primary target for PRRSV infection in pigs. At least six cellular molecules have been described so far as putative receptors for PRRSV, and they include heparan sulfate, vimentin, CD151, sialoadhesin (CD169; siglec-1), dendritic cell-specific intercellul...
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#1Matthew A. Kappes (ARS: Agricultural Research Service)H-Index: 8
#2Kay S. Faaberg (ARS: Agricultural Research Service)H-Index: 41
Abstract Porcine reproductive and respiratory disease virus (PRRSV) has the intrinsic ability to adapt and evolve. After 25 years of study, this persistent pathogen has continued to frustrate efforts to eliminate infection of herds through vaccination or other elimination strategies. The purpose of this review is to summarize the research on the virion structure, replication and recombination properties of PRRSV that have led to the extraordinary phenotype and genotype diversity that exists worl...
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