Methods to Investigate miRNA Function: Focus on Platelet Reactivity.

Published on Apr 1, 2021in Thrombosis and Haemostasis4.379
· DOI :10.1055/S-0040-1718730
Alix Garcia3
Estimated H-index: 3
(University of Geneva),
Sylvie Dunoyer-Geindre16
Estimated H-index: 16
(University of Geneva)
+ 3 AuthorsPierre Fontana31
Estimated H-index: 31
(Geneva College)
Sources
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs modulating protein production. They are key players in regulation of cell function and are considered as biomarkers in several diseases. The identification of the proteins they regulate, and their impact on cell physiology, may delineate their role as diagnostic or prognostic markers and identify new therapeutic strategies. During the last 3 decades, development of a large panel of techniques has given rise to multiple models dedicated to the study of miRNAs. Since plasma samples are easily accessible, circulating miRNAs can be studied in clinical trials. To quantify miRNAs in numerous plasma samples, the choice of extraction and purification techniques, as well as normalization procedures, are important for comparisons of miRNA levels in populations and over time. Recent advances in bioinformatics provide tools to identify putative miRNAs targets that can then be validated with dedicated assays. In vitro and in vivo approaches aim to functionally validate candidate miRNAs from correlations and to understand their impact on cellular processes. This review describes the advantages and pitfalls of the available techniques for translational research to study miRNAs with a focus on their role in regulating platelet reactivity.
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