Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry.

Published on Oct 27, 2020in Journal of Separation Science2.878
· DOI :10.1002/JSSC.202000842
Jingwei Li1
Estimated H-index: 1
(SPU: Shenyang Pharmaceutical University),
Miao Wang9
Estimated H-index: 9
(SPU: Shenyang Pharmaceutical University)
+ 3 AuthorsMin Zhao8
Estimated H-index: 8
(SPU: Shenyang Pharmaceutical University)
Sources
Abstract
Duzhong Jiangya Tablet is a hypotensive drug. In this study, High-performance liquid chromatography-fourier transform-ion cyclotron resonance-mass spectrometry technology was used to quickly identify its chemical composition. SinoChrom ODS-BP column (250mm×4.6mm, 5μm) was used. The mobile phase was acetonitrile(A)-0.1% formic acid solution(B). The flow rate was 1 mL·min-1 . Extracted ion chromatogram was used to analyze the samples in positive and negative ion modes. Based on the obtained accurate mass spectrometry information such as excimer ions and fragment ions, combined with the reference substance and the reference literature, to identify the chemical constituents of Duzhong Jiangya Tablet. A total of 131 compounds were identified, including 4 types of penylpropanoids, 6 types of phenylethanoid glycosides, 10 types of organic acids, 14 types of iridoids, 12 types of lignans, 18 types of alkaloids, 7 types of coumarins, and 60 kinds of flavonoids. This established method can quickly and efficiently identify chemical constituents in Duzhong Jiangya Tablet, and lay a foundation for the research on the efficacy and quality of this traditional Chinese medicine, and provide a reference for the characterization of the chemical constituents of other traditional Chinese medicine preparations. This article is protected by copyright. All rights reserved.
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