61P CRISPR/Cas9 and bioinformatics approaches revealed new regulators of pancreatic cancer cells platinum sensitivity

Pancreatic adenocarcinoma (PDAC) is one of the most lethal cancers characterized by multidrug resistance. Identification of new predictive biomarkers of PDAC is urgently needed. MIA PaCa-2 pancreatic adenocarcinoma cells were used for CRISPR/Cas9 screening with sgRNA libraries against 1) cell cycle+nuclear protein genes and 2) the whole genome. The cells were treated with sublethal concentrations of cisplatin or oxaliplatin for at least 9 cell...