Epitope

Disease

Coronavirus disease 2019 (COVID-19)

Immunity

Biology

Pandemic

Computer science

Gene

Cloning (programming)

Antigen

Vaccination

Immunology

Genetics

Medicine

Computational biology

In silico

Peptide vaccine

Population

Infectious disease (medical specialty)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Environmental health

Virology

Programming language

Immune system

Pathology

Scientific Reports

COVID-19 pandemic has resulted in 16,114,449 cases with 646,641 deaths from the 217 countries, or territories as on July 27th 2020. Due to multifaceted issues and challenges in the implementation of the safety and preventive measures, inconsistent coordination between societies-governments and most importantly lack of specific vaccine to SARS-CoV-2, the spread of the virus that initially emerged at Wuhan is still uprising after taking a heavy toll on human life. In the present study, we mapped immunogenic epitopes present on the four structural proteins of SARS-CoV-2 and we designed a multi-epitope peptide based vaccine that, demonstrated a high immunogenic response with a vast application on world’s human population. On codon optimization and in-silico cloning, we found that candidate vaccine showed high expression in E. coli and immune simulation resulted in inducing a high level of both B-cell and T-cell mediated immunity. The results predicted that exposure of vaccine by administrating three injections si...

Designing a multi-epitope peptide based vaccine against SARS-CoV-2