Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope

Published on Sep 28, 2020in Optics Express3.669
· DOI :10.1364/OE.404869
Peng Lin6
Estimated H-index: 6
(BU: Boston University),
Hongli Ni1
Estimated H-index: 1
(BU: Boston University)
+ 5 AuthorsJi-Xin Cheng83
Estimated H-index: 83
(BU: Boston University)
Operable under ambient light and providing chemical selectivity, stimulated Raman scattering (SRS) microscopy opens a new window for imaging molecular events on a human subject, such as filtration of topical drugs through the skin. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores.
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