Proline-rich domain of human ALIX contains multiple TSG101-UEV interaction sites and forms phosphorylation-mediated reversible amyloids

Significance Human ALIX is involved in many essential cellular processes. The carboxy (C)-terminal proline-rich domain (PRD) of ALIX, which interacts with multiple partners, has eluded analysis owing to the presence of polyproline stretches that hinder recombinant protein production. By dividing it into two fragments, the amino (N)- and the C-terminal portions, we carry out a detailed biophysical investigation of ALIX-PRD. The disordered...