Coupling p-Hydroxybenzoate Hydroaxylase with the Photoresponsive Nanozyme for Universal Dehydrogenase-Based Bioassays

Herein, an innovative dehydrogenase invoked tri-enzyme cascade has been successfully constructed and utilized as a powerful and universal colorimetric strategy for dehydrogenase-based bioassays. Specifically, the glucose-6-phosphatedehydrogenase (G6PD) catalyzes the specific dehydrogenation of glucose-6-phosphate (G-6-P) by consuming the oxidized form of β-nicotinamide adenine dinucleotide phosphate (NADP+) to generate NADPH. And the formed NADPH...