Preincubation with a low-dose hydrogen peroxide enhances anti-oxidative stress ability of BMSCs

Objective To investigate the effects of low-concentration hydrogen peroxide pretreatment on the anti-oxidative stress of the bone marrow mesenchymal stem cells (BMSCs). Methods Rabbit BMSCs were isolated and cultured by density gradient centrifugation combined with the adherence method. Then, the third generation of well-grown BMSCs was continuously treated with 50-μM hydrogen peroxide (H 2 O 2 ) for 8 h as the optimal pretreatment concentration...