Engineering

Biodegradation

Nanotechnology

Organic chemistry

Enzyme

Chemistry

Materials science

Polyvinylidene fluoride

Immobilized enzyme

Membrane

Chemical engineering

Chromatography

Carbon nanotube

Waste management

Biochemistry

Laccase

Nanocomposite

Wastewater

Chemosphere

The presence of pharmaceutical micropollutants in water and wastewater is considered a serious environmental issue. To eliminate these pollutants, biodegradation of pharmaceuticals using enzymes such as laccase, is proposed as a green method. In this study, immobilized laccase was used for the removal of two model pharmaceutical compounds, carbamazepine and diclofenac. Polyvinylidene fluoride (PVDF) membrane modified with multi-walled carbon nanotubes (MWCNTs) were synthesized as a tailor-made support for enzyme immobilization. Covalently immobilized laccase from Trametes hirsuta exhibited remarkable activity and activity recovery of 4.47 U/cm2 and 38.31%, respectively. The results also indicated improvement in the operational and thermal stability of the immobilized laccase compared to free laccase. Finally, by using immobilized laccase in a mini-membrane reactor, removal efficiencies of 27% in 48 h and 95% in 4 h were obtained for carbamazepine and diclofenac, respectively. The findings suggest that immobilized laccase on PVDF/MWCNT membranes is a promising catalyst for large-scale water and wastewater treatment which is also compatible with existing treatment facilities.

Pharmaceuticals removal by immobilized laccase on polyvinylidene fluoride nanocomposite with multi-walled carbon nanotubes