Rapid immunoassays for detection of anabolic nortestosterone in dietary supplements

An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under optimal experimental conditions, the most sensitive ELISA achieved the IC50 and the limit of detection values of 6.41 and 0.09 ng/ml, respectively. The assay...