Characterization, antibiofilm and biocompatibility properties of chitosan hydrogels loaded with silver nanoparticles and ampicillin: an alternative protection to central venous catheters.

Published on Dec 1, 2020in Colloids and Surfaces B: Biointerfaces4.389
· DOI :10.1016/J.COLSURFB.2020.111292
Montserrat Lopez-Carrizales2
Estimated H-index: 2
(Universidad Autónoma de San Luis Potosí),
E. Mendoza-Mendoza7
Estimated H-index: 7
(Universidad Autónoma de San Luis Potosí)
+ 9 AuthorsFidel Martinez-Gutierrez13
Estimated H-index: 13
(Universidad Autónoma de San Luis Potosí)
Source
Abstract
Abstract The purpose of this study was to generate novel chitosan hydrogels (CHs) loaded with silver nanoparticles (AgNPs) and ampicillin (AMP) to prevent early formation of biofilms. AgNPs and CHs were characterized by UV-Vis, DLS, TEM, rheology, FT-IR, Raman, and SEM. The antibiofilm effect of the formulations was investigated against four multidrug-resistant and extensively drug-resistant pathogens using a colony biofilm, a high cell density and gradients model. Also, their hemostatic properties and cytotoxic effect were evaluated. Rheology results showed that CHs with AgNPs and AMP are typical non-Newtonian pseudoplastic fluids. The CH with 25 ppm of AgNPs and 50 ppm AMP inhibited the formation of biofilms of Acinetobacter baumannii, Enterococcus faecium and Staphylococcus epidermidis, while a ten-fold increase of the antimicrobial’s concentration was needed to inhibit the biofilm of the β-lactamase positive Enterobacter cloacae. Further, CH with 250 ppm of AgNPs and 500 ppm AMP showed anticoagulant effect, and it was shown that all formulations were biocompatible. Besides to previous reports that described the bioadhesion properties of chitosan, these results suggest that AgNPs and AMP CHs loaded could be used as prophylactic treatment in patients with central venous catheter (CVC), inhibiting the formation of biofilms in their early stages, in addition to their anticoagulant effect and biocompatibility, those properties could keep the functionality of CVC helping to prevent complications such as sepsis and thrombosis.
📖 Papers frequently viewed together
56 Citations
21 Citations
28 Citations
References41
Newest
#1Carlos Bermúdez‐Jiménez (Universidad Autónoma de San Luis Potosí)H-Index: 2
#2Nereyda Niño-Martínez (Universidad Autónoma de San Luis Potosí)H-Index: 12
Last. Gabriel Alejandro Martínez-Castañón (Universidad Autónoma de San Luis Potosí)H-Index: 19
view all 7 authors...
: Biofilms are matrices synthesized by bacteria containing polysaccharides, DNA, and proteins. The development of biofilms in infectious processes can induce a chronic inflammatory response that may progress to the destruction of tissues. The treatment of biofilms is difficult because they serve as a bacterial mechanism of defense and high doses of antibiotics are necessary to treat these infections with limited positive results. It has been demonstrated that photothermal therapy using gold nano...
6 CitationsSource
#1Gong Jingni (Fujian Agriculture and Forestry University)H-Index: 7
#2Wang Lin (Fujian Agriculture and Forestry University)H-Index: 13
Last. Jie Pang (Fujian Agriculture and Forestry University)H-Index: 19
view all 8 authors...
Abstract Thermosensitive hydrogels are known for their application potential in a wide range of fields. In this study, a novel thermosensitive hydrogel was prepared under specific physical gelling conditions using konjac glucomannan (KGM) and gum tragacanth (GT). The rheology results showed that KGM/GT mixed hydrocolloids are a typical non-Newtonian pseudoplastic fluid. They have significant elastic properties: G′ was higher than G″ at all hydrocolloid ratios. Increasing the GT concentration res...
16 CitationsSource
#1Vaishali Pawar (IITB: Indian Institute of Technology Bombay)H-Index: 5
#2Mukesh Dhanka (IITB: Indian Institute of Technology Bombay)H-Index: 6
Last. Rohit Srivastava (IITB: Indian Institute of Technology Bombay)H-Index: 29
view all 3 authors...
Abstract Hydrogels are excellent drug delivery systems for the treatment of chronic wound infections. However, the problem of high burst release still remains a challenge that needs to be tackled. In terms of antibiotic release from the hydrogels, as the drug payload depletes it could act as a substrate for bacterial seeding which can create a life-threatening condition. Therefore, to provide the sustained effect of an antibiotic at the localized site via hydrogel matrix, we prepared a chitosan ...
25 CitationsSource
#1A. Hernandez-Rangel (CINVESTAV)H-Index: 4
Last. Gabriel Luna-Bárcenas (CINVESTAV)H-Index: 27
view all 9 authors...
Abstract We report the synthesis and in vitro evaluation of dual-function chitosan-silver nanoparticles (CTS-AgNPs) films with potential applications as wound dressings. We attempted to formulate nanocomposite films with appropriate AgNPs concentrations to simultaneously display antibacterial activity and suitability for cell culture. Nanocomposites were obtained by CTS-mediated in situ chemical reduction of AgNO 3 . Circular-shape AgNPs (sizes ca . 7–50 nm) well distributed within the CTS matri...
22 CitationsSource
#1Zhang Hu (GDOU: Guangdong Ocean University)H-Index: 7
#2Sitong Lu (GDOU: Guangdong Ocean University)H-Index: 3
Last. Lei Yang (GDOU: Guangdong Ocean University)H-Index: 9
view all 7 authors...
Hemorrhea is one of the major problems in war, trauma care, and surgical operation that threaten the life of the injured and patients. As a novel polymeric hemostatic agent, biodegradable chitosan can stop bleeding through a variety of approaches. In this paper, chitosan with various molecular parameters was prepared from chitin as raw material through deacetylation, oxidative degradation, hydrophilic modification, and salt formation reactions. The influence of different polymer parameters on th...
24 CitationsSource
#1Yajuan Xie (Lanzhou University)H-Index: 1
#2Xiaozhu Liao (Lanzhou University)H-Index: 5
Last. Zengjie Fan (Lanzhou University)H-Index: 16
view all 5 authors...
Abstract Poor mechanical properties of chitosan hydrogels limit their applications as the wound dressing. To overcome this drawback, we abandoned the traditional glacial acetic acid method but adopted a LiOH/urea solvent system to synthesize chitosan hydrogel. Then reductive Ag nanoparticles were integrated into the chitosan hydrogel networks, aiming at reinforcing the mechanical properties and improving the antibacterial properties of chitosan hydrogel. The synthesized hydrogels were subsequent...
64 CitationsSource
#1Hamid Hamedi (NCSU: North Carolina State University)H-Index: 5
#2Sara Moradi (NCSU: North Carolina State University)H-Index: 6
Last. Alan E. Tonelli (NCSU: North Carolina State University)H-Index: 56
view all 4 authors...
Abstract Advanced development of chitosan hydrogels has led to new drug delivery systems that can release their active ingredients in response to environmental stimuli. This review considers more recent investigation of chitosan hydrogel preparations and the application of these preparations for drug delivery in wound dressings. Applications and structural characteristics of different types of active ingredients, such as growth factors, nanoparticles, nanostructures, and drug loaded chitosan hyd...
243 CitationsSource
#1Montserrat Lopez-Carrizales (Universidad Autónoma de San Luis Potosí)H-Index: 2
#2Karla Itzel Velasco (Universidad Autónoma de San Luis Potosí)H-Index: 1
Last. Fidel Martinez-Gutierrez (Universidad Autónoma de San Luis Potosí)H-Index: 13
view all 7 authors...
The increase in the prevalence of bacterial resistance to antibiotics has become one of the main health problems worldwide, thus threatening the era of antibiotics most frequently used in the treatment of infections. The need to develop new therapeutic strategies against multidrug resistant microorganisms, such as the combination of selected antimicrobials, can be considered as a suitable alternative. The in vitro activities of two groups of conventional antimicrobial agents alone and in combina...
20 CitationsSource
#1Sandra SchulzH-Index: 1
#2Manfred F. Maitz (Leibniz Institute for Neurobiology)H-Index: 5
Last. Carsten Werner (TUD: Dresden University of Technology)H-Index: 76
view all 5 authors...
Advanced blood contacting biomaterials are designed to combine antiseptic and anticoagulant functionalities. Here, we present a new in vitro methodology for the analysis of bacterial adhesion and growth after the preceding human whole blood incubation of the tested materials. Poly(styrene) surfaces as well as thrombin-responsive and non-responsive poly(ethylene glycol)–heparin hydrogel coatings, with and without silver functionalization, were analyzed with this approach using freshly drawn human...
4 CitationsSource
#1Bushra Jamil (CUI: COMSATS Institute of Information Technology)H-Index: 21
#2Muhammad Imran (CUI: COMSATS Institute of Information Technology)H-Index: 101
AbstractUnderstanding the interplay between bacterial pathogens and antimicrobials is a key to realize the control over infections causing morbidity and mortality. An important current issue of contemporary medicine and microbiology is the search for new strategies for adequate therapy of infectious diseases associated with rapidly emerging multidrug-resistant (MDR) pathogens. Recently, a great deal of progress has been made in the field of nanobiotechnology towards the development of various na...
11 CitationsSource
Cited By3
Newest
#1Yingshuo Xiong (Ludong University)H-Index: 1
#2Xiaoran Zhang (Ludong University)H-Index: 1
Last. Changmei Sun (Ludong University)
view all 9 authors...
Due to their outstanding properties, bioadhesive hydrogels have been extensively studied by researchers in recent years. By designing the properties of bioadhesive hydrogels, they can be reasonably applied to biomedicine. Therefore, this practicality has prompted the birth of many bioadhesive hydrogels with excellent properties. In this review, by studying the progress in the field of bioadhesive hydrogels, we summarized the properties of hydrogels in bioadhesion, including adhesion, biocompatib...
Source
#1Arianna Pompilio (University of Chieti-Pescara)H-Index: 18
#2Daniela Scribano (Sapienza University of Rome)H-Index: 12
Last. Cecilia Ambrosi (OU: Open University)H-Index: 13
view all 6 authors...
Bacterial biofilms are a serious public-health problem worldwide. In recent years, the rates of antibiotic-resistant Gram-negative bacteria associated with biofilm-forming activity have increased worrisomely, particularly among healthcare-associated pathogens. Acinetobacter baumannii is a critically opportunistic pathogen, due to the high rates of antibiotic resistant strains causing healthcare-acquired infections (HAIs). The clinical isolates of A. baumannii can form biofilms on both biotic and...
Source
#1Marcos A. Polinarski (UFPR: Federal University of Paraná)H-Index: 1
#2Ana L. B. Beal (UFPR: Federal University of Paraná)H-Index: 1
Last. Helton José Alves (UFPR: Federal University of Paraná)H-Index: 14
view all 7 authors...
1 CitationsSource