Evaluation of Exosome Proteins by on‐Bead Flow Cytometry

Exosomes, recently re‐named “small extracellular vesicles” or “sEV,” are emerging as an intercellular communication system. Quantification of the molecular cargo exosomes carry by on‐bead flow cytometry is needed for defining their role in information transfer and in human disease. Exosomes (sEV) isolated from cell supernatants or plasma of cancer patients by size‐exclusion chromatography were captured by biotinylated antibodies specific for...