Ultrafast visual nucleic acid detection with CRISPR/Cas12a and rapid PCR in single capillary

Volume: 326, Pages: 128618 - 128618
Published: Jan 1, 2021
Abstract
• We have proposed an ultrafast nucleic acid detection method that could be accomplished in 5∼10 min. • This method is based on one-pot rapid PCR amplification and CRISPR/Cas cleavage system. • The detection process is quite simple and detection result is visible. • This method is feasible for field applications with powerless instruments. • This method is robust, specific and could detect target at single molecule level. Rapid and sensitive...
Paper Details
Title
Ultrafast visual nucleic acid detection with CRISPR/Cas12a and rapid PCR in single capillary
Published Date
Jan 1, 2021
Volume
326
Pages
128618 - 128618
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