A thiamine-triggered fluormetric assay for acetylcholinesterase activity and inhibitor screening based on oxidase-like activity of MnO2 nanosheets

Acetylcholinesterase (AChE) plays an essential role in biological signal transmission, the aberrant expression of which could cause diverse neurodegenerative diseases. Herein, based on the oxidase-like activity of manganese dioxide nanosheets (MnO2 NSs), we found that MnO2 NSs could directly oxidize thiamine into intensely fluorescent thiochrome without the need of peroxides. When AChE was introduced, acetylthiocholine could be hydrolyzed to...