Transcriptome profiling reveals signaling conditions dictating human spermatogonia fate in vitro

Significance The ability to culture human spermatogonial stem cells (SSCs) in vitro is critical to develop SSC therapeutic approaches to treat male infertility. To achieve this goal, it is important to define molecular pathways in human SSCs. Toward this end, we developed an approach to purify human primitive undifferentiated spermatogonia (uSPG) highly enriched in SSCs. Comparative RNA-sequencing analysis of these primitive uSPG versus...