Use of Chou’s 5-steps rule to predict the subcellular localization of gram-negative and gram-positive bacterial proteins by multi-label learning based on gene ontology annotation and profile alignment

To date, many proteins generated by large-scale genome sequencing projects are still uncharacterized and subject to intensive investigations by both experimental and computational means. Knowledge of protein subcellular localization (SCL) is of key importance for protein function elucidation. However, it remains a challenging task, especially for multiple sites proteins known to shuttle between cell compartments to perform their proper...