A novel fluorometric aptasensor based on carbon nanocomposite for sensitive detection of Escherichia coli O157:H7 in milk

Escherichia coli O157:H7 is an extremely serious foodborne pathogen accounting for a vast number of hospitalizations. In this system, a simple, rapid, and safe compound method was developed based on carbonyl iron powder (CIP) and multiwalled carbon nanotubes (MWCNT). Then, the CIP@MWCNT-based aptasensor was constructed by strong π-stacking between nanocomposite and aptamer, single-strand DNA, causing fluorescent quenching of the dye-labeled...