Gene

Engineering

Biotechnology

Bioenergy

Catalysis

Biochemical engineering

Yeast

Yarrowia

Lipid accumulation

Lipid metabolism

Biodiesel

Biofuel

Biochemistry

Metabolic engineering

Biology

Bioresource Technology

Current energy security and climate change policies encourage the development and utilization of bioenergy. Oleaginous yeasts provide a particularly attractive platform for the sustainable production of biofuels and industrial chemicals due to their ability to accumulate high amounts of lipids. In particular, microbial lipids in the form of triacylglycerides (TAGs) produced from renewable feedstocks have attracted considerable attention because they can be directly used in the production of biodiesel and oleochemicals analogous to petrochemicals. As an oleaginous yeast that is generally regarded as safe, Yarrowia lipolytica has been extensively studied, with large amounts of data on its lipid metabolism, genetic tools, and genome sequencing and annotation. In this review, we highlight the newest strategies for increasing lipid accumulation using metabolic engineering and summarize the research advances on the overaccumulation of lipids in Y. lipolytica. Finally, perspectives for future engineering approaches are proposed.

Metabolic engineering for increased lipid accumulation in Yarrowia lipolytica – A Review