Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.

Published on Jun 16, 2020in Journal of Separation Science2.878
· DOI :10.1002/JSSC.202000268
Renata Zátopková1
Estimated H-index: 1
,
Zeineb Aturki29
Estimated H-index: 29
,
Petr Bednář17
Estimated H-index: 17
Sources
Abstract
A nano-liquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile phase additive such as cyclodextrin. The chiral separation of eriodictyol, naringenin and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin. The influence of the composition of the mobile phase, the length of the capillary column, the flow rate on the chiral recognition were investigated. At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30 % methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively. Precision, linearity and sensitivity of the method was tested. Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 μg/mL and 7.5-12.5 μg/mL, respectively. The method was used for the determination of the diastereomeric composition of the flavanone -7-O-glycosides in Citrus juices after solid-phase extraction procedure. This article is protected by copyright. All rights reserved.
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