Expression, Purification, and Evaluation of Anti-IL-2Rα Antibody Secreted by Leishmania tarentolae

Producing a functional anti-IL-2Rα antibody in Leishmania tarentolae, a trypanosomatid protozoan non-pathogenic to human, is a cost-effective and safe alternative for the production of therapeutic recombinant proteins. Expression cassettes encoding heavy and light chains of the anti-IL-2Rα antibody were cloned into two separate pLEXSY vectors. The plasmids were then used to transfect L. tarentolae laboratory strain p10 and stable expression of a...