Development and validation of RP-HPLC method for estimation of fisetin in rat plasma

• RP-HPLC method for fisetin was developed in rat plasma. • The developed method was validated as per ICH guidelines. • Method was found linear in the concentration range of 25–125 ng/mL. • Recovery above 95% and % RSD less than 2 indicated about accuracy and precision of method. • Absence of interfering peaks at the retention times of fisetin and quercetin indicated method's specificity. Fisetin is an important phyto-flavonoid that possesses...