SIRT1–NOX4 signaling axis regulates cancer cachexia

Published on Jul 6, 2020in Journal of Experimental Medicine11.743
· DOI :10.1084/JEM.20190745
Aneesha Dasgupta8
Estimated H-index: 8
(UNMC: University of Nebraska Medical Center),
Surendra K. Shukla17
Estimated H-index: 17
(Eppley Institute for Research in Cancer and Allied Diseases)
+ 27 AuthorsPankaj K. Singh32
Estimated H-index: 32
(UNMC: University of Nebraska Medical Center)
: Approximately one third of cancer patients die due to complexities related to cachexia. However, the mechanisms of cachexia and the potential therapeutic interventions remain poorly studied. We observed a significant positive correlation between SIRT1 expression and muscle fiber cross-sectional area in pancreatic cancer patients. Rescuing Sirt1 expression by exogenous expression or pharmacological agents reverted cancer cell-induced myotube wasting in culture conditions and mouse models. RNA-seq and follow-up analyses showed cancer cell-mediated SIRT1 loss induced NF-κB signaling in cachectic muscles that enhanced the expression of FOXO transcription factors and NADPH oxidase 4 (Nox4), a key regulator of reactive oxygen species production. Additionally, we observed a negative correlation between NOX4 expression and skeletal muscle fiber cross-sectional area in pancreatic cancer patients. Knocking out Nox4 in skeletal muscles or pharmacological blockade of Nox4 activity abrogated tumor-induced cachexia in mice. Thus, we conclude that targeting the Sirt1-Nox4 axis in muscles is an effective therapeutic intervention for mitigating pancreatic cancer-induced cachexia.
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