Novel strategies of Raman imaging for monitoring the therapeutic benefit of temozolomide in glioblastoma

We developed a label-free Raman method for monitoring the therapeutic benefit of temozolomide in glioblastoma by whole-cell biochemical hyperspectral imaging for detecting molecular processes occurring in normal and tumorous brain cells at the level of isolated organelles. Our approach allows biochemical compositional mapping of lipid droplets, mitochondria, and nucleus in single cells, monitoring intracellular alterations upon using different...