Visualizing Newly Synthesized RNA by Bioorthogonal Labeling-Primed DNA Amplification
Abstract
Monitoring RNA synthesis and spatial distribution can help to understand its role in physiology and diseases. However, visualizing newly synthesized RNA in single cells remains a great challenge. Here, we developed a bioorthogonal labeling-primed DNA amplification strategy to visualize newly synthesized RNA in single cells. The new bioorthogonal N6-allyladenosine nucleoside was prepared to metabolically label cellular newly synthesized RNAs....
Paper Details
Title
Visualizing Newly Synthesized RNA by Bioorthogonal Labeling-Primed DNA Amplification
Published Date
May 15, 2020
Journal
Volume
92
Issue
12
Pages
8444 - 8449
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