Original paper

Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval

Volume: 17, Issue: 5, Pages: 531 - 540
Published: May 1, 2020
Abstract
Single-molecule localization microscopy is a powerful tool for visualizing subcellular structures, interactions and protein functions in biological research. However, inhomogeneous refractive indices inside cells and tissues distort the fluorescent signal emitted from single-molecule probes, which rapidly degrades resolution with increasing depth. We propose a method that enables the construction of an in situ 3D response of single emitters...
Paper Details
Title
Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval
Published Date
May 1, 2020
Volume
17
Issue
5
Pages
531 - 540
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