Improved fractionation method demonstrates phosphorylated Akt is localized to the cytoplasm in LβT2 cells

Cellular processes controlling proliferation, migration, differentiation and adherence require signal transduction processes. Subcellular compartmentalization of signaling proteins controls their temporal and spatial activity, and can be assessed by subcellular fractionation. However, most fractionation protocols are not effective for certain cell lines as the integrity of the samples produced are often compromised by cross‐contamination. Here,...