Development of a highly sensitive chemiluminescence immunoassay for detection of aggrecanase-generated args aggrecan fragments in serum

Purpose: Cartilage metabolism and turnover is a hallmark of Osteoarthritis (OA), and can be assessed by blood- or urine-based markers of extracellular matrix protein (ECMP) degradation. Levels of such markers may reflect disease activity and as such be applied in drug development as pharmacodynamic markers. Aggrecan, a major ECMP of articular cartilage, is degraded by ADAMTS-5, which is upregulated and activated in OA. One of the well-described...