Integration of the metabolome and transcriptome reveals the resistance mechanism to low nitrogen in wild soybean seedling roots

Published on Jul 1, 2020in Environmental and Experimental Botany4.027
· DOI :10.1016/J.ENVEXPBOT.2020.104043
Danping Liu1
Estimated H-index: 1
(Northeast Normal University),
Mingxia Li3
Estimated H-index: 3
(Northeast Normal University)
+ 1 AuthorsLianxuan Shi5
Estimated H-index: 5
(Northeast Normal University)
Abstract Wild soybean is the progenitor of cultivated soybean that has excellent low-nitrogen (N) tolerance. This study revealed the mechanism of resistance to low N in wild soybean seedling roots by analyzing the differences in the physiology, metabolome and transcriptome. Under low-N stress, low-N-tolerant wild soybean seedling roots underwent less growth inhibition, producing longer roots that had a higher carbon (C)/N ratio and C, NO3−, Fe2+, Zn2+, Ca2+, K+ and B3+ contents compared with common wild soybean. The comparison of the metabolomes and transcriptomes of two genotypes of wild soybean revealed that low-N-tolerant wild soybean reduced its energy consumption by decreasing the synthesis of energy-consuming amino acids. In addition, it enhanced the synthesis of proline and secondary metabolites to resist low-N stress. Enhancing the metabolism of shikimic acid was a unique mechanism involved in low-N tolerance of the low-N-tolerant wild soybean. Moreover, some genes involved in flavonoid synthesis were identified. This study could provide a basis for the investigation and application of wild resources in soybean breeding.
📖 Papers frequently viewed together
4 Citations
28 Citations
24 Citations
#1Pandurang R. Divte (IARI: Indian Agricultural Research Institute)H-Index: 1
#2Poonam Yadav (IARI: Indian Agricultural Research Institute)H-Index: 4
Last. Bhupinder Singh (IARI: Indian Agricultural Research Institute)H-Index: 63
view all 5 authors...
Abstract Two separate experiments were conducted with four bread and two durum wheat cultivars, to test a hypothesis that high ethylene production will alter phytosiderophore production and release dynamics either directly, by competitive partitioning of SAM, or indirectly by causing changes in the root system architecture under iron deficiency. A significant decline in the leaf greenness index confirmed the Fe deficiency response across the wheat cultivars. Further, a significantly higher root ...
9 CitationsSource
#1Hengyou Zhang (UNCC: University of North Carolina at Charlotte)H-Index: 13
#2Farida Yasmin (UNCC: University of North Carolina at Charlotte)H-Index: 1
Last. Bao-Hua Song (UNCC: University of North Carolina at Charlotte)H-Index: 19
view all 3 authors...
The legume family (Fabaceae) is the third-largest flowering family with over 18 000 species worldwide that are rich in proteins, oils, and nutrients. However, the production potential of legume-derived food cannot meet increasing global demand. Wild legumes represent a large group of wild species adaptive to diverse habitats and harbor rich genetic diversity for the improvement of the agronomic, nutritional, and medicinal values of the domesticated legumes. Accumulating evidence suggests that th...
11 CitationsSource
#1Mingxia Li (Northeast Normal University)H-Index: 3
#2Jie XuH-Index: 1
Last. Lianxuan Shi (Northeast Normal University)H-Index: 4
view all 8 authors...
Abstract Wild soybean (Glycine soja) and cultivated soybean (Glycine max) belong to the subgenus Soja. We investigated the photosynthetic activity, mineral nutrition and metabolomics of the salt-tolerant wild soybean (W2), salt-sensitive wild soybean (W1) and cultivated soybean (C) in the early flowering stage, with a focus on the physiological and cellular metabolism-related differences among Soja to reveal the adaptive mechanisms. The photosynthetic activity of W2 was greater than that of W1 a...
3 CitationsSource
#1Muhammad Riaz (HAU: Huazhong Agricultural University)H-Index: 62
#2Lei Yan (HAU: Huazhong Agricultural University)H-Index: 7
Last. Cuncang Jiang (HAU: Huazhong Agricultural University)H-Index: 14
view all 6 authors...
Abstract Aluminum is a major limiting abiotic factor for plant growth and productivity on acidic soils. The primary disorder of aluminum toxicity is the rapid cessation of root elongation. The root apex is the most sensitive part of this organ. Although significant literature evidence and hypothesis exist on aluminum toxicity, the explicit mechanism through which aluminum ceases root growth is still indefinable. The mechanisms of tolerance in plants have been the focus of intense research. Some ...
31 CitationsSource
#1Lu Huang (ZJU: Zhejiang University)H-Index: 7
#2Liuhui Kuang (ZJU: Zhejiang University)H-Index: 6
Last. Guoping Zhang (ZJU: Zhejiang University)H-Index: 72
view all 6 authors...
Abstract Sea barley (Hordeum marinum) is a wild species belonging to halophytes, while other Hordeum species are glycophytes. In this study, we aimed to understand molecular differences of transcriptomic and metabolomic responses and uncover complex mechanisms underlying salt tolerance between H. marinum and H. vulgare. Physiological studies revealed that sea barley accession H559 had less growth inhibition, higher root K+ concentration, lower shoot Na+ accumulation and higher shoot osmotic pote...
17 CitationsSource
#1Mingxia Li (Northeast Normal University)H-Index: 1
#2Jingshu Xu (Northeast Normal University)H-Index: 2
Last. Lianxuan Shi (Northeast Normal University)H-Index: 1
view all 7 authors...
Abstract Nitrogen is an essential macronutrient for plants and the common limiting factor for crop productivity worldwide. An effective approach to combat N deficiency and overuse is to understand the mechanism of low-nitrogen tolerance in plants and develop low-nitrogen-tolerant crop cultivars. Wild soybean has a high tolerance to poor environmental conditions, but, until now, no study has illustrated the mechanism of low-nitrogen tolerance at a metabolomic level. In this study, the photosynthe...
28 CitationsSource
#1Janice Kofsky (UNCC: University of North Carolina at Charlotte)H-Index: 3
#2Hengyou Zhang (UNCC: University of North Carolina at Charlotte)H-Index: 13
Last. Bao-Hua Song (UNCC: University of North Carolina at Charlotte)H-Index: 19
view all 3 authors...
There is a considerable demand for crop improvement, especially considering the increasing growth of world population, continuing climatic fluctuations, and rapidly evolving plant pests and pathogens. Crop wild relatives hold great potential in providing beneficial alleles for crop improvement. Wild soybean, Glycine soja (Siebold & Zucc.), the wild ancestor to the domesticated soybean (Glycine max (L.) Merr.), harbors a high level of genetic variation. Research on G. soja has been largely devote...
25 CitationsSource
#1Yanliang WangH-Index: 11
#2Erik LysøeH-Index: 17
Last. Jihong Liu ClarkeH-Index: 15
view all 8 authors...
: Roots and root-released organic anions play important roles in uptake of phosphorus (P), an essential macronutrient for food production. Oat, ranking sixth in the world's cereal production, contains valuable nutritional compounds and can withstand poor soil conditions. Our aim was to investigate root transcriptional and metabolic responses of oat grown under P-deficient and P-sufficient conditions. We conducted a hydroponic experiment and measured root morphology and organic anion exudation, a...
29 CitationsSource
#1Jian Liang (CUHK: The Chinese University of Hong Kong)H-Index: 1
#2Jun-Xian He (CUHK: The Chinese University of Hong Kong)H-Index: 20
Abstract Nitrogen (N) is a major nutrient of plants but often a limiting factor for plant growth and crop yield. To adapt to N deficiency, plants have evolved adaptive responses including accumulation of anthocyanins. However, it is still unclear whether the accumulated anthocyanins are part of the components of plant tolerance under low N stress. Here, we demonstrate that low N-induced anthocyanins contribute substantially to the low N tolerance of Arabidopsis thaliana . pap1-1 , a mutant defec...
23 CitationsSource
#1Peter R. Ryan (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 58
Acid soils (pH <5.0) continue to limit the yields of Australia’s major crops and restrict their cultivation. These soils pose various abiotic stresses that restrict or affect plant growth in different ways. Chief among these stresses is aluminium (Al3+) toxicity, which inhibits root growth. Soil acidification can occur naturally but certain agricultural practices accelerate the process. The most effective management practice for slowing and reversing acidification is the application of lime (cal...
20 CitationsSource
Cited By7
Abstract null null Nitrogen (N) is an essential element for plant growth and development. In this study, physiological, transcriptome and metabolite analyses of apple leaves and roots were compared under different N conditions. The results indicated that different N stresses influenced plant growth indexes, the content of soil plant analytical development (SPAD) value and photosynthesis in apple seedlings. The content of hydrogen peroxide (H2O2), and the activities of superoxide dismutase (SOD),...
#1Wang YanjieH-Index: 3
#2Wang DemeiH-Index: 5
Last. Chang XuhongH-Index: 5
view all 7 authors...
Nitrogen (N) supplementation is essential to the yield and quality of bread wheat (Triticum aestivum L.). The impact of N-deficiency on wheat at the seedling stage has been previously reported, but the impact of distinct N regimes applied at the seedling stage with continuous application on filling and maturing wheat grains is lesser-known, despite the filling stage being critical for final grain yield and flour quality. Here we compared the phenotype characteristics including grain yield, grain...
#1Nikolai P. Bityutskii (SPbU: Saint Petersburg State University)H-Index: 7
#2Kirill Yakkonen (SPbU: Saint Petersburg State University)H-Index: 7
Last. Konstantin N. SemenovH-Index: 16
view all 6 authors...
The unique properties of carbon-based nanomaterials, including fullerenol, have attracted great interest in agricultural and environmental applications. Iron (Fe) is an essential micronutrient for major metabolic processes, for which a shortage causes chlorosis and reduces the yield of many crops cultivated worldwide. In the current study, the metabolic responses of Cucumis sativus (a Strategy I plant) to fullerenol treatments were investigated depending on the Fe status of plants. Cucumber plan...
#1Li He (JGSU: Jinggangshan University)
#2Li Teng (Haida: Hainan University)
Last. Li Liao (Haida: Hainan University)
view all 7 authors...
Axonopus compressus also known as carpet grass is a robust, stoloniferous grass that can grow in minimal fertilization and resists well to abiotic and biotic stresses including low nitrogen (LN) stress. This study aimed at characterizing the agro-morphological and metabolome responses to LN in carpet grass leaves. Under LN stress, carpet grass increased yellowness of leaves and root dry matter while reduced turf quality and shoot dry weight. The metabolome comparison between samples from optimum...
#1Qi Sun (NEAU: Northeast Agricultural University)H-Index: 2
#2Haoran Lu (NEAU: Northeast Agricultural University)H-Index: 1
Last. Qiang Li (NEAU: Northeast Agricultural University)H-Index: 5
view all 8 authors...
Nitrogen is one of the essential elements for plant growth. Wild soybeans (Glycine soja) have strong abilities to survive in harsh and barren environments, and hence become ideal plant model for studying plant adaptability to low nitrogen (LN) conditions. In this study, we analyzed and compared the transcriptomes of wild soybean subjected to LN treatments. We totally identified 1095 (681 up and 414 down) and 5490 (2998 up and 2492 down) differentially expressed genes (DEGs) in the aerial parts (...
#1Li WeiH-Index: 1
#2Mu PengH-Index: 2
Last. Deyue Yu
view all 11 authors...
Wild soybean germplasm is distributed widely in China, particularly in Heilongjiang Province. In this study, 242 wild soybean accessions from four agricultural divisions in Heilongjiang Province were evaluated based on six qualitative and eight quantitative traits. Results showed that a large amount of variation occurred in these evaluated traits. Abundant qualitative traits included the wild type (78.51%), purple flowers (90.50%), needle leaf (39.26%), black seed (83.88%), brown hilum (52.07%),...
#1Xiaomin Liu (NWAFU: Northwest A&F University)H-Index: 2
#2Tengteng Gao (NWAFU: Northwest A&F University)H-Index: 6
Last. Chao Li (NWAFU: Northwest A&F University)H-Index: 1
view all 8 authors...
Abstract Dopamine plays numerous physiological roles in plants. We explored its role in the regulation of growth, nutrient absorption, and response to nitrogen (N) deficiency in Malus hupehensis Rehd. Under low N condition, plant growth slowed, and the net photosynthetic rates, chlorophyll contents, and maximal quantum yield of PSII (Fv/Fm) decreased significantly. However, the application of 100 μmol L−1 exogenous dopamine significantly reduced the inhibition of low N stress on plant growth. In...
2 CitationsSource