Active Ingredients and Anti-Arthritic Mechanisms of Ba-Wei-Long-Zuan Granule Revealed by 1 H-NMR-Based Metabolomics Combined with Network Pharmacology Analysis.

Published on Jun 1, 2020in Chemistry & Biodiversity2.039
· DOI :10.1002/CBDV.202000122
Gang Fan9
Estimated H-index: 9
(CDUTCM: Chengdu University of Traditional Chinese Medicine),
Qi Li7
Estimated H-index: 7
(CDUTCM: Chengdu University of Traditional Chinese Medicine)
+ 5 AuthorsLei‐Lei Du1
Estimated H-index: 1
(CDUTCM: Chengdu University of Traditional Chinese Medicine)
Ba-Wei-Long-Zuan granule (BWLZ) is a traditional herbal preparation. It has been widely used for the treatment of rheumatoid arthritis (RA). However, its active ingredients and mechanisms of action are still unclear. The present study aims to reveal the active compounds and anti-arthritic mechanisms of BWLZ against collagen-induced arthritis (CIA) by using 1H NMR-based metabolomics, molecular docking and network pharmacology methods. After 30 days of administration, BWLZ could effectively improve the metabolic disorders in CIA rats. The anti-arthritic effect of BWLZ was related to its restoration of 16 disturbed serum metabolites. Molecular docking and network analysis showed that 20 compounds present in BWLZ could act on multiple targets. Among them, coclaurine and hesperidin showed the highest hit rates for target proteins related to both metabolic regulation and RA, indicating that these two compounds might be potential active ingredients of BWLZ. Moreover, pathway enrichment analysis suggested that the anti-arthritic mechanisms of BWLZ might be attributed to its network regulation of several biological processessuch as steroid hormone biosynthesis, mTOR signaling pathway, alanine, aspartate and glutamate metabolism, and synthesis and degradation of ketone bodies. These results provide further evidence for the anti-arthritic properties of BWLZ and are beneficial for its quality control and clinical application. The potential targets and biological processes found in this study may provide valuable information for further studying the molecular mechanisms of BWLZ against RA. In addition, our work provides new insights for revealing the active ingredients and regulatory mechanisms of complex herbal preparations.
📖 Papers frequently viewed together
20193.12RSC Advances
4 Authors (Zhen Jin, ..., Gang Cao)
1 Citations
10 Citations
3 Citations
#1Cao Jianhua (Shanxi University)H-Index: 1
#2Duan Yahui (Shanxi University)H-Index: 1
Last. Zhenyu Li (Shanxi University)H-Index: 25
view all 9 authors...
Abstract Ethnopharmacological relevance Naozhenning granule (NZN), a widely traditional Chinese medicine (TCM) prescription with a long history of clinical, which is mainly used in the treatment of concussion, cerebral post-traumatic syndrome, consists of Di Huang (Radix of Rehmannia glutinosa (Gaertn.) DC.), Dang Gui (Radix of Angelica sinensis (Oliv.) Diels), Chen Pi (Pericarpium of Citrus reticulata Blanco), Dan shen (Radix of Salvia Miltiorrhiza Bunge.), Di Long (Pheretima aspergillum (E. Pe...
3 CitationsSource
#1Su-Ling Zeng (CPU: China Pharmaceutical University)H-Index: 11
#2Shang-Zhen Li (CPU: China Pharmaceutical University)H-Index: 3
Last. E-Hu Liu (CPU: China Pharmaceutical University)H-Index: 30
view all 9 authors...
Metabolic syndrome (MetS) is intricately linked to dysregulation of gut microbiota and host metabolomes. Here, we first find that a purified citrus polymethoxyflavone-rich extract (PMFE) potently ameliorates high-fat diet (HFD)–induced MetS, alleviates gut dysbiosis, and regulates branched-chain amino acid (BCAA) metabolism using 16S rDNA amplicon sequencing and metabolomic profiling. The metabolic protective effects of PMFE are gut microbiota dependent, as demonstrated by antibiotic treatment a...
34 CitationsSource
#1ChongZhe Pei (SJTU: Shanghai Jiao Tong University)H-Index: 1
#2Xiaoyan Wang (Fudan University)H-Index: 1
Last. Shu Meng (SJTU: Shanghai Jiao Tong University)H-Index: 14
view all 5 authors...
Endothelial progenitor cells (EPCs) have the potential to repair damaged blood vessels and promote angiogenesis. Smoking, an important risk factor for cardiovascular diseases, is associated with impaired functions of EPCs. However, the underlying mechanisms remain unclear. The aim of the study was to investigate the effects of cigarette smoke extract (CSE) on autophagy and dysfunction of EPCs and the involvement of galectin-3 in its effects. EPCs were treated with 8% CSE for 24 h (without affect...
69 CitationsSource
#1Hai-Jiao Li (CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 3
#2Chuan‐Tao Zhang (CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 1
Last. Gang Fan (CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 1
view all 8 authors...
: Bawei Longzuan granule (BLG) is a representative Zhuang medicine preparation. The present work aims to characterize the chemical constituents of BLG and evaluate its anti-arthritic activity. The major chemical constituents of BLG were tentatively identified by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), which revealed the presence of some alkaloids (e. g., magnoflorine, sinomenine and nitidine) and flavonoids (e. g., hesperidin, diosmin ...
6 CitationsSource
#1Małgorzata Radwan-Oczko (Wrocław Medical University)H-Index: 11
#2Irena Duś-Ilnicka (Wrocław Medical University)H-Index: 2
Last. Claus RasmussenH-Index: 1
view all 5 authors...
AIM: Rheumatoid arthritis (RA) and periodontal diseases (PD) are common chronic, inflammatory, destructive and progressive diseases that may have similar pathophysiological mechanisms and risk factors. RA affects more than 1.5% of the world's population, with a higher percentage of females than males. PD is present in around 20% of the population and has multifactorial etiology. The purpose of this study is to describe patients' self-reported oral health and the association with RA disease activ...
4 CitationsSource
#1Dandan TianH-Index: 1
#2Jianhua SunH-Index: 1
Last. Zhang ZhengchenH-Index: 2
view all 10 authors...
The aim of this study was to investigate the protective effect of Traditional Chinese medicine Shuangwu Zhentong Capsule (SZC) on collagen-induced arthritis (CIA) and the possible mechanisms. Sixty...
1 CitationsSource
#1Wei Peng (CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 1
#1Wei Peng (CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 9
Last. Gang Fan (CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 9
view all 7 authors...
San-Huang-Xie-Xin decoction (SHXXD), composed of Rhei Radix et Rhizoma, Coptidis Rhizoma, and Scutellariae Radix, is a representative antipyretic and detoxifying prescription in traditional Chinese medicine. In this study, we investigated the antistress effects and underlying mechanisms of San-Huang-Xie-Xin decoction (SHXXD) on restraint-stressed mice by 1H NMR-based metabolomics combined with biochemistry assay. A total of 48 male mice (5 weeks old, 18-22 g) were divided randomly into 6 groups ...
5 CitationsSource
#1Adel M. Al-ghazzawi (King Khalid University)H-Index: 3
Two alkaloids, belonging to benzylisoquinoline alkaloids, were isolated from Annona squamosa. One of these alkaloids, 6, 7-dimethoxy-1-(α-hydroxy-4-methoxybenzyl)-2-methyl-1, 2, 3, 4-tetrahydroisoquinoline, was isolated for the first time from natural sources, while, the alkaloid, Coclaurine was known in Annona squamosa L. Moreover, the isolated alkaloids tested for the anti-cancer activities on various cell lines (HepG-2, MCF-7, and HCT-116). Open image in new window
5 CitationsSource
AbstractBackgroundRheumatoid arthritis affects 1% of the world's population and its current treatment options are costly. There are not enough studies that evaluated the efficacy and safety of anti-inflammatory drugs medications used to reduce rheumatoid arthritis's symptoms. This study will evaluat
8 CitationsSource
#1Talita Sartori (USP: University of São Paulo)H-Index: 4
#2Guilherme Galvão dos Santos (USP: University of São Paulo)H-Index: 3
Last. Ricardo Ambrósio Fock (USP: University of São Paulo)H-Index: 20
view all 7 authors...
The immune system is essential for the control and elimination of infections, and macrophages are cells that act as important players in orchestrating the various parts of the inflammatory/immune response. Amino acids play important role in mediating functionality of the inflammatory response, especially mediating macrophages functions and cytokines production. We investigated the influence of glutamine, taurine and their association on the modulation of inflammatory pathway markers in macrophag...
12 CitationsSource
Cited By3
#1Yumeng Zhang (SPU: Shenyang Pharmaceutical University)H-Index: 4
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Miao Wang (SPU: Shenyang Pharmaceutical University)H-Index: 9
view all 6 authors...
Hepatic fibrosis (HF) is a typical consequence of various chronic liver diseases, and there is still no ideal drug for its treatment. Yinchen Wuling Powder (YCWLP), a famous traditional Chinese medicine prescription, is effective for the treatment of icteric hepatitis, hepatic fibrosis, non-alcoholic fatty liver disease and other liver diseases in clinical practices, however, the underlying mechanisms of YCWLP on HF is still unclear. In this study, 1H NMR and MS-based metabolomics analysis along...
Rheumatoid arthritis (RA) is an autoimmune disorder affecting a vast variety of the population. The onset of RA as well as the development of systematic immunization is affected by both genetic and environmental risk factors. This review aims to point out the role of natural products in the management of RA, focusing on the reports of basic research (in vitro and animal studies) emphasizing the antioxidant and anti-inflammatory properties considered in the field of RA. A systematic screening of ...
#1Yanqing Song (JLU: Jilin University)H-Index: 8
#2Tingting HuH-Index: 2
Last. Xiaoyu Qu (JLU: Jilin University)H-Index: 9
view all 10 authors...
Abstract Cisplatin (CDDP)-induced acute kidney injury (AKI) limits the therapeutic use of CDDP, which urgently needs to be addressed. Our previous study demonstrated that astragaloside IV (AS IV), an active compound of the traditional Chinese herb Astragalus membranaceus, alleviated CDDP-induced AKI. To explore the mechanism, we performed a metabolomics study to explore the altered metabolic pathways and screen for sensitive biomarkers. Twenty-four rats were randomly divided into three groups, w...
1 CitationsSource