Directed evolution of adenine base editors with increased activity and therapeutic application.

Volume: 38, Issue: 7, Pages: 892 - 900
Published: Apr 13, 2020
Abstract
The foundational adenine base editors (for example, ABE7.10) enable programmable A•T to G•C point mutations but editing efficiencies can be low at challenging loci in primary human cells. Here we further evolve ABE7.10 using a library of adenosine deaminase variants to create ABE8s. At NGG protospacer adjacent motif (PAM) sites, ABE8s result in ~1.5× higher editing at protospacer positions A5-A7 and ~3.2× higher editing at positions A3-A4 and...
Paper Details
Title
Directed evolution of adenine base editors with increased activity and therapeutic application.
DOI
Published Date
Apr 13, 2020
Journal
Volume
38
Issue
7
Pages
892 - 900
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