Rapid modification of hemoglobin heme to form enhanced peroxidase-like activity for colorimetric assay

Modification of proteins is widespread throughout nature responsible for the vast biodiversity. In this study, we developed a facile method to rapid and selectively modify heme of hemoglobin (Hb) with tartaric acid to form complex (TA-Hb), which exhibits strong peroxidase-like activity comparable to the native horseradish peroxidase (HRP). The structural change of Hb accompanied by the formation of TA-Hb complex was validated by UV-vis...