Internal medicine

Biotechnology

In vivo

Glycogen debranching enzyme

Biology

Endocrinology

Glycogen synthase

Chemistry

Insulin resistance

Glycogen

Steatosis

Insulin

Glucose homeostasis

Homeostasis

Medicine

Glucokinase

Proceedings of the National Academy of Sciences

Significance Hepatic glycogen synthesis plays a critical role in maintaining normal glucose homeostasis; however, the rate-controlling step regulating this process is unknown. Applying metabolic control analysis in vivo, we show that the regulation of insulin-stimulated hepatic glycogen synthesis under both normal and pathophysiological conditions of fatty liver-associated hepatic insulin resistance is controlled at the glucokinase (GCK) step through GCK translocation.

Metabolic control analysis of hepatic glycogen synthesis in vivo